INTRODUCTION: The aim of the present study was to investigate the effect of MTAD on the differentiation of osteoblast-like cells. METHODS: The cell viability assay was performed to evaluate the cytotoxicity of MTAD on MC3T3-E1 and periodontal ligament cells at the various concentrations. The bone sialoprotein (BSP) gene expression was also examined by real-time polymerase chain reaction. RESULTS: MTAD exhibited a lower cytotoxicity compared with other intracanal irrigants and medication. The MC3T3-E1 cells treated with H2O2 showed a decrease in the alkaline phosphatase (ALP) activity by 40% on day 14 compared with the control group at the concentration of 50 microg/mlL. No significant difference in the ALP activity was observed between MTAD and control group. Furthermore, MTAD and Ca(OH)2 paste did not change in BSP gene expression in MC3T3-E1 cells on day 21. CONCLUSIONS: These results suggested that MTAD is a less cytotoxic irrigant and does not affect differentiation into osteoblasts compared with other intracanal irrigants, such as H2O2, NaOCl, ethylenediaminetetraacetic acid, and chlorhexidine. Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
INTRODUCTION: The aim of the present study was to investigate the effect of MTAD on the differentiation of osteoblast-like cells. METHODS: The cell viability assay was performed to evaluate the cytotoxicity of MTAD on MC3T3-E1 and periodontal ligament cells at the various concentrations. The bone sialoprotein (BSP) gene expression was also examined by real-time polymerase chain reaction. RESULTS: MTAD exhibited a lower cytotoxicity compared with other intracanal irrigants and medication. The MC3T3-E1 cells treated with H2O2 showed a decrease in the alkaline phosphatase (ALP) activity by 40% on day 14 compared with the control group at the concentration of 50 microg/mlL. No significant difference in the ALP activity was observed between MTAD and control group. Furthermore, MTAD and Ca(OH)2 paste did not change in BSP gene expression in MC3T3-E1 cells on day 21. CONCLUSIONS: These results suggested that MTAD is a less cytotoxic irrigant and does not affect differentiation into osteoblasts compared with other intracanal irrigants, such as H2O2, NaOCl, ethylenediaminetetraacetic acid, and chlorhexidine. Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Authors: Mohammad Ali Saghiri; Abbas Delvarani; Payman Mehrvarzfar; Mohsen Nikoo; Mehrdad Lotfi; Kasra Karamifar; Kamal Asgar; Sahar Dadvand Journal: Saudi Dent J Date: 2011-03-09