BACKGROUND: Activation-induced cytidine deaminase (AID) is a B-cell-specific DNA mutator that plays a key role in the formation of the secondary antibody repertoire in germinal center B cells. In the search for binding partners, protein coimmunoprecipitation assays are often performed, generally with agarose beads. METHODOLOGY/PRINCIPAL FINDINGS: We found that, regardless of whether cell lysates containing exogenous or endogenous AID were examined, one of two mouse AID forms bound to agarose alone. CONCLUSIONS/SIGNIFICANCE: These binding characteristics may be due to the known post-translational modifications of AID; they may also need to be considered in coimmunoprecipitation experiments to avoid false-positive results.
BACKGROUND:Activation-induced cytidine deaminase (AID) is a B-cell-specific DNA mutator that plays a key role in the formation of the secondary antibody repertoire in germinal center B cells. In the search for binding partners, protein coimmunoprecipitation assays are often performed, generally with agarose beads. METHODOLOGY/PRINCIPAL FINDINGS: We found that, regardless of whether cell lysates containing exogenous or endogenous AID were examined, one of two mouseAID forms bound to agarose alone. CONCLUSIONS/SIGNIFICANCE: These binding characteristics may be due to the known post-translational modifications of AID; they may also need to be considered in coimmunoprecipitation experiments to avoid false-positive results.
Authors: Vasco Barreto; Bernardo Reina-San-Martin; Almudena R Ramiro; Kevin M McBride; Michel C Nussenzweig Journal: Mol Cell Date: 2003-08 Impact factor: 17.970
Authors: Almudena R Ramiro; Mila Jankovic; Thomas Eisenreich; Simone Difilippantonio; Selina Chen-Kiang; Masamichi Muramatsu; Tasuku Honjo; André Nussenzweig; Michel C Nussenzweig Journal: Cell Date: 2004-08-20 Impact factor: 41.582
Authors: Kevin M McBride; Vasco Barreto; Almudena R Ramiro; Pete Stavropoulos; Michel C Nussenzweig Journal: J Exp Med Date: 2004-04-26 Impact factor: 14.307