| Literature DB >> 2011129 |
T Ota1, S Higashi, H Suzuki, S Eto.
Abstract
An inhibition enzyme-linked immunosorbent assay (inhibition-ELISA) was developed for the quantitative determination of human IgG (Gm) allotypes using rabbit anti-Gm antisera, alkaline-phosphatase-conjugated goat anti-rabbit IgG and, as the calibrant, purified human myeloma proteins possessing the relevant Gm allotype. The assay is reproducible and can detect as little as 10 ng/ml of G1m(a), G2m(n) or G3m(st), and 100 ng/ml of G1m(f) or G3m(g). Using this assay, the "gene dosage effect" and "allelic balance" in healthy Japanese were studied.Entities:
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Year: 1991 PMID： 2011129 DOI： 10.1016/0161-5890(91)90086-y
Source DB: PubMed Journal: Mol Immunol ISSN： 0161-5890 Impact factor: 4.407