BACKGROUND: Thymic stromal lymphopoietin (TSLP) is elevated in airway inflammatory diseases such as asthma and triggers dendritic cell-mediated activation of Th2 inflammatory responses. Although allergic chronic sinusitis is a Th2 inflammatory disease of the upper airway, the mechanism underlying the predominance of Th2 responses still has to be clarified. We investigated the expression of TSLP in cytokine-treated nasal polyp fibroblasts. METHODS: Fibroblast lines were established from nasal polyp tissues. Their expression of TSLP mRNA was evaluated by real-time reverse-transcription polymerase chain reaction. The amount of TSLP in the supernatants was measured by enzyme-linked immunosorbent assay. RESULTS: Nasal polyp fibroblasts have the capacity to produce TSLP in response to stimulation by tumor necrosis factor (TNF) alpha. Combined stimulation with TNF-alpha + a Th2 cytokine (IL-4 or IL-13) was synergistic for TSLP production by the nasal polyp fibroblasts. This response was time and dose dependent. The TNF-alpha + Th2 cytokine (IL-4 or IL-13)-induced TSLP production was strongly inhibited by interferon gamma but not by IL-10. CONCLUSION: These results suggest that nasal polyp fibroblasts play a role in the development and regulation of Th2-type inflammation in the upper airway by producing TSLP.
BACKGROUND:Thymic stromal lymphopoietin (TSLP) is elevated in airway inflammatory diseases such as asthma and triggers dendritic cell-mediated activation of Th2 inflammatory responses. Although allergic chronic sinusitis is a Th2 inflammatory disease of the upper airway, the mechanism underlying the predominance of Th2 responses still has to be clarified. We investigated the expression of TSLP in cytokine-treated nasal polyp fibroblasts. METHODS: Fibroblast lines were established from nasal polyp tissues. Their expression of TSLP mRNA was evaluated by real-time reverse-transcription polymerase chain reaction. The amount of TSLP in the supernatants was measured by enzyme-linked immunosorbent assay. RESULTS: Nasal polyp fibroblasts have the capacity to produce TSLP in response to stimulation by tumor necrosis factor (TNF) alpha. Combined stimulation with TNF-alpha + a Th2 cytokine (IL-4 or IL-13) was synergistic for TSLP production by the nasal polyp fibroblasts. This response was time and dose dependent. The TNF-alpha + Th2 cytokine (IL-4 or IL-13)-induced TSLP production was strongly inhibited by interferon gamma but not by IL-10. CONCLUSION: These results suggest that nasal polyp fibroblasts play a role in the development and regulation of Th2-type inflammation in the upper airway by producing TSLP.
Authors: William W Carroll; Rodney J Schlosser; Brendan P O'Connell; Zachary M Soler; Jennifer K Mulligan Journal: Int Forum Allergy Rhinol Date: 2016-01-11 Impact factor: 3.858
Authors: Geovanny F Perez; Krishna Pancham; Shehlanoor Huseni; Diego Preciado; Robert J Freishtat; Anamaris M Colberg-Poley; Eric P Hoffman; Mary C Rose; Gustavo Nino Journal: Eur Respir J Date: 2014-06-25 Impact factor: 16.671
Authors: William R Proctor; Mala Chakraborty; Aaron M Fullerton; Midhun C Korrapati; Pauline M Ryan; Kenrick Semple; Jeffrey C Morrison; Julia D Berkson; Lynette S Chea; Qian Yang; Albert P Li; Rosanne Spolski; Erin E West; Yrina Rochman; Warren J Leonard; Mohammed Bourdi; Lance R Pohl Journal: Hepatology Date: 2014-05-20 Impact factor: 17.425
Authors: Stephen L Ball; Monika I Suwara; Lee A Borthwick; Janet A Wilson; Derek A Mann; Andrew J Fisher Journal: Ann Otol Rhinol Laryngol Date: 2014-12-23 Impact factor: 1.547
Authors: Claus Bachert; Ruby Pawankar; Luo Zhang; Chaweewan Bunnag; Wytske J Fokkens; Daniel L Hamilos; Orathai Jirapongsananuruk; Robert Kern; Eli O Meltzer; Joaquim Mullol; Robert Naclerio; Renata Pilan; Chae-Seo Rhee; Harumi Suzaki; Richard Voegels; Michael Blaiss Journal: World Allergy Organ J Date: 2014-10-27 Impact factor: 4.084