BACKGROUND AND OBJECTIVE: The role of Mycobacterium avium ss paratuberculosis (MAP) in the etiopathology of Crohn's disease (CD) remains controversial, because of conflicting reports demonstrating the presence of MAP-specific insertion sequence from intestinal biopsy tissues of patients clinically diagnosed for the disease. The present study was carried out to investigate the presence of MAP DNA in the intestinal tissues of CD patients to ascertain the relevance of MAP in Indian patients with CD. METHODS: Patients diagnosed as CD at our institute were recruited. Healthy individuals without inflammatory bowel disease served as controls. Mucosal biopsy specimens were collected from ileum and colon in duplicates and subjected to histopathological examination and polymerase chain reaction (PCR) amplification. Total DNA (81 CD patients, 85 healthy individuals) and total RNA (12 CD patients, 12 healthy individuals) isolated from tissue specimens was used for amplification of MAP-specific IS900 by nested PCR. RESULTS: MAP-specific IS900 DNA and RNA could not be detected by nested PCR in the intestinal tissues of any patient with CD. CONCLUSION: Our results do not support the etiological role of MAP in the pathogenesis of CD in Indian patients.
BACKGROUND AND OBJECTIVE: The role of Mycobacterium avium ss paratuberculosis (MAP) in the etiopathology of Crohn's disease (CD) remains controversial, because of conflicting reports demonstrating the presence of MAP-specific insertion sequence from intestinal biopsy tissues of patients clinically diagnosed for the disease. The present study was carried out to investigate the presence of MAP DNA in the intestinal tissues of CDpatients to ascertain the relevance of MAP in Indian patients with CD. METHODS:Patients diagnosed as CD at our institute were recruited. Healthy individuals without inflammatory bowel disease served as controls. Mucosal biopsy specimens were collected from ileum and colon in duplicates and subjected to histopathological examination and polymerase chain reaction (PCR) amplification. Total DNA (81 CDpatients, 85 healthy individuals) and total RNA (12 CDpatients, 12 healthy individuals) isolated from tissue specimens was used for amplification of MAP-specific IS900 by nested PCR. RESULTS: MAP-specific IS900 DNA and RNA could not be detected by nested PCR in the intestinal tissues of any patient with CD. CONCLUSION: Our results do not support the etiological role of MAP in the pathogenesis of CD in Indian patients.
Authors: Anli Gao; Joseph Odumeru; Melinda Raymond; Steven Hendrick; Todd Duffield; Lucy Mutharia Journal: Can J Vet Res Date: 2009-01 Impact factor: 1.310
Authors: Georgina L Hold; Megan Smith; Charlie Grange; Euan Robert Watt; Emad M El-Omar; Indrani Mukhopadhya Journal: World J Gastroenterol Date: 2014-02-07 Impact factor: 5.742
Authors: Timo Rath; Martin Roderfeld; Sonja Blöcher; Annika Rhode; Tina Basler; Ömer Akineden; Amir Abdulmawjood; Jörg M Halwe; Ralph Goethe; Michael Bülte; Elke Roeb Journal: BMC Gastroenterol Date: 2011-04-08 Impact factor: 3.067
Authors: John P Bannantine; Judith R Stabel; Elise A Lamont; Robert E Briggs; Srinand Sreevatsan Journal: Front Microbiol Date: 2011-07-26 Impact factor: 5.640