Ping Dong1, Yi Yang, Wei-ping Wang. 1. Children's Hospital, Pediatrics Department of Shanghai Medical College, Fudan University, Shanghai, PR China.
Abstract
AIM: The effects of intestinal bifidobacteria on the development of immunity in early life were explored. METHODS: Neonatal SD rats born and housed under strict barrier systems were fed from birth with sufficient antibiotics (bifidobacteria minimisation group) or supplemented daily with 1x10(10) colony-forming units of live Bifidobacterium longum (bifidobacteria supplementation group). Relevant indices of immune development were determined at one, three and six weeks old. RESULTS: Compared to the control group, minimisation of the intestinal bifidobacteria delayed maturation of dendritic cells in Peyer's Patches and the development of T cells in the thymus, increased IL-4 secretion in the plasma, down-regulated IL-12, IL-10 mRNA and the interferon-gamma/IL-4 mRNA ratio in intestinal mucosa, decreased interferon-gamma mRNA in cultured peripheral blood mononuclear cells (PBMCs), and reduced immunoglobulin-M production in cultured PBMCs. Conversely, supplementation with bifidobacteria promoted dendritic cell maturation in Peyer's Patches, up-regulated IL-12, IL-10, interferon-gamma mRNA and the interferon-gamma/IL-4 ratio in intestinal mucosa, increased interferon-gamma gene expression in cultured PBMCs, and raised immunoglobulin-M secretion in cultured PBMCs. CONCLUSIONS: Intestinal bifidobacteria could promote the maturation of dendritic cells and its expression of IL-12 locally in the gut, influence the development of T cells in the thymus, favour the development of T-helper cell type 1 response by increasing the local and systemic expression of interferon-gamma and ensure the intestinal regulatory T cell response by promoting the local expression of IL-10. In addition, they enhance antibody synthesis by PBMCs, thereby affecting the development of both the gut and systemic immunity in early life.
AIM: The effects of intestinal bifidobacteria on the development of immunity in early life were explored. METHODS: Neonatal SD rats born and housed under strict barrier systems were fed from birth with sufficient antibiotics (bifidobacteria minimisation group) or supplemented daily with 1x10(10) colony-forming units of live Bifidobacterium longum (bifidobacteria supplementation group). Relevant indices of immune development were determined at one, three and six weeks old. RESULTS: Compared to the control group, minimisation of the intestinal bifidobacteria delayed maturation of dendritic cells in Peyer's Patches and the development of T cells in the thymus, increased IL-4 secretion in the plasma, down-regulated IL-12, IL-10 mRNA and the interferon-gamma/IL-4 mRNA ratio in intestinal mucosa, decreased interferon-gamma mRNA in cultured peripheral blood mononuclear cells (PBMCs), and reduced immunoglobulin-M production in cultured PBMCs. Conversely, supplementation with bifidobacteria promoted dendritic cell maturation in Peyer's Patches, up-regulated IL-12, IL-10, interferon-gamma mRNA and the interferon-gamma/IL-4 ratio in intestinal mucosa, increased interferon-gamma gene expression in cultured PBMCs, and raised immunoglobulin-M secretion in cultured PBMCs. CONCLUSIONS: Intestinal bifidobacteria could promote the maturation of dendritic cells and its expression of IL-12 locally in the gut, influence the development of T cells in the thymus, favour the development of T-helper cell type 1 response by increasing the local and systemic expression of interferon-gamma and ensure the intestinal regulatory T cell response by promoting the local expression of IL-10. In addition, they enhance antibody synthesis by PBMCs, thereby affecting the development of both the gut and systemic immunity in early life.
Authors: Ayelet Sivan; Leticia Corrales; Nathaniel Hubert; Jason B Williams; Keston Aquino-Michaels; Zachary M Earley; Franco W Benyamin; Yuk Man Lei; Bana Jabri; Maria-Luisa Alegre; Eugene B Chang; Thomas F Gajewski Journal: Science Date: 2015-11-05 Impact factor: 47.728
Authors: M C C Canesso; L Lemos; T C Neves; F M Marim; T B R Castro; É S Veloso; C P Queiroz; J Ahn; H C Santiago; F S Martins; J Alves-Silva; E Ferreira; D C Cara; A T Vieira; G N Barber; S C Oliveira; A M C Faria Journal: Mucosal Immunol Date: 2017-12-20 Impact factor: 7.313
Authors: Maria A Johansson; Ylva M Sjögren; Jan-Olov Persson; Caroline Nilsson; Eva Sverremark-Ekström Journal: PLoS One Date: 2011-08-01 Impact factor: 3.240
Authors: Jennifer T Smilowitz; Jackelyn Moya; Melissa A Breck; Chelsea Cook; Annette Fineberg; Kathleen Angkustsiri; Mark A Underwood Journal: BMC Pediatr Date: 2017-05-30 Impact factor: 2.125