Literature DB >> 2009920

Leishmania: genus identification based on a specific sequence of the 18S ribosomal RNA sequence.

S R Uliana1, M H Affonso, E P Camargo, L M Floeter-Winter.   

Abstract

The analysis of PvuII restriction patterns of Leishmania spp. and Trypanosoma spp. genomic DNA showed genus distinctive profiles. A specific PvuII site was detected in the 5' domain of 18S ribosomal DNA of Leishmania. A 20-mer oligonucleotide encompassing this PvuII region was synthesized. This sequence, when utilized as probe, on short exposures of dot tests, detected 10(3) whole promastigotes of all Leishmania species analyzed but did not hybridize with T. cruzi or human nucleic acids. Two other oligonucleotides were synthesized to be used as primers for amplification through polymerase chain reaction of the 18S ribosomal DNA region containing the PvuII site. The probes described may be useful for the detection of Leishmania spp. under clinical and epidemiological trials.

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Year:  1991        PMID： 2009920     DOI： 10.1016/0014-4894(91)90133-h

Source DB:  PubMed          Journal:  Exp Parasitol        ISSN： 0014-4894            Impact factor:   2.011


  22 in total

Review 1.  [Molecular biological techniques in the diagnosis of tropical parasitic diseases].

Authors:  R Felleisen; M Q Klinkert
Journal:  Naturwissenschaften       Date:  1992-11

2.  Rapid identification of causative species in patients with Old World leishmaniasis.

Authors:  P Minodier; R Piarroux; F Gambarelli; C Joblet; H Dumon
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3.  Evaluation of PCR for diagnosis of visceral leishmaniasis.

Authors:  O F Osman; L Oskam; E E Zijlstra; N C Kroon; G J Schoone; E T Khalil; A M El-Hassan; P A Kager
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

4.  Defining DNA-based operational taxonomic units for microbial-eukaryote ecology.

Authors:  David A Caron; Peter D Countway; Pratik Savai; Rebecca J Gast; Astrid Schnetzer; Stefanie D Moorthi; Mark R Dennett; Dawn M Moran; Adriane C Jones
Journal:  Appl Environ Microbiol       Date:  2009-07-10       Impact factor: 4.792

Review 5.  DNA probes and PCR for diagnosis of parasitic infections.

Authors:  J B Weiss
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

6.  PCR and in vitro cultivation for detection of Leishmania spp. in diagnostic samples from humans and dogs.

Authors:  A Mathis; P Deplazes
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

7.  Use of PCR for diagnosis of post-kala-azar dermal leishmaniasis.

Authors:  O F Osman; L Oskam; N C Kroon; G J Schoone; E T Khalil; A M El-Hassan; E E Zijlstra; P A Kager
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

8.  New PCR assay using glucose-6-phosphate dehydrogenase for identification of Leishmania species.

Authors:  Tiago M Castilho; Jeffrey Jon Shaw; Lucile M Floeter-Winter
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

9.  Practical approach for typing strains of Leishmania infantum by microsatellite analysis.

Authors:  Béatrice Bulle; Laurence Millon; Jean-Mathieu Bart; Montserrat Gállego; Françoise Gambarelli; Montserrat Portús; Lee Schnur; Charles L Jaffe; Salceda Fernandez-Barredo; José María Alunda; Renaud Piarroux
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

10.  Comparison of PCR with direct examination of bone marrow aspiration, myeloculture, and serology for diagnosis of visceral Leishmaniasis in immunocompromised patients.

Authors:  R Piarroux; F Gambarelli; H Dumon; M Fontes; S Dunan; C Mary; B Toga; M Quilici
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948
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