BACKGROUND: We have recently profiled inflammatory gene expression in acute appendicitis (AA) and identified a set of genes that are overexpressed in AA. The purpose of this study was to determine whether serum levels of a subset of proteins coded for by these overexpressed genes could differentiate patients with AA from those with other causes of abdominal pain and whether the serum levels of these proteins correlate with the histologic severity of appendicitis. MATERIALS AND METHODS: Serum samples were obtained from a convenience sample of children between 1 and 21 y of age who presented to the emergency department (ED) with symptoms/signs of AA. Patients were assigned to the proven appendicitis (AA) or nonappendicitis (control) group based on histologic findings, chart review, and follow-up phone calls. The serum levels of haptoglobin, granulocyte colony stimulating factor (GCSF), interleukin 8 (IL-8), and C-reactive protein (CRP) were compared between groups. For patients with histologically proven appendicitis, a histologic severity score was assigned and correlated with the levels of potential serum biomarkers. RESULTS: thirty-two patients were enrolled; 23 patients (72%) had AA. Serum levels of CRP and GCSF were significantly different between AA and control groups (7.0 versus 0.8, P=0.01 and 104.2 versus 58.7, P=0.03, respectively.) In patients with AA, there was significant correlation between GCSF serum levels and the appendicitis severity score [r=0.537, P=0.02]. CONCLUSIONS: GCSF serum levels can distinguish between patients with AA and controls. GCSF may prove to be a useful adjunctive test in the diagnosis and staging of acute appendicitis.
BACKGROUND: We have recently profiled inflammatory gene expression in acute appendicitis (AA) and identified a set of genes that are overexpressed in AA. The purpose of this study was to determine whether serum levels of a subset of proteins coded for by these overexpressed genes could differentiate patients with AA from those with other causes of abdominal pain and whether the serum levels of these proteins correlate with the histologic severity of appendicitis. MATERIALS AND METHODS: Serum samples were obtained from a convenience sample of children between 1 and 21 y of age who presented to the emergency department (ED) with symptoms/signs of AA. Patients were assigned to the proven appendicitis (AA) or nonappendicitis (control) group based on histologic findings, chart review, and follow-up phone calls. The serum levels of haptoglobin, granulocyte colony stimulating factor (GCSF), interleukin 8 (IL-8), and C-reactive protein (CRP) were compared between groups. For patients with histologically proven appendicitis, a histologic severity score was assigned and correlated with the levels of potential serum biomarkers. RESULTS: thirty-two patients were enrolled; 23 patients (72%) had AA. Serum levels of CRP and GCSF were significantly different between AA and control groups (7.0 versus 0.8, P=0.01 and 104.2 versus 58.7, P=0.03, respectively.) In patients with AA, there was significant correlation between GCSF serum levels and the appendicitis severity score [r=0.537, P=0.02]. CONCLUSIONS:GCSF serum levels can distinguish between patients with AA and controls. GCSF may prove to be a useful adjunctive test in the diagnosis and staging of acute appendicitis.
Authors: Augusto Zani; Warwick J Teague; Simon A Clarke; Munther J Haddad; Sanjeev Khurana; Thomas Tsang; Ramesh M Nataraja Journal: Pediatr Surg Int Date: 2017-04-29 Impact factor: 1.827
Authors: Manne Andersson; Marie Rubér; Christina Ekerfelt; Hanna Björnsson Hallgren; Gunnar Olaison; Roland E Andersson Journal: World J Surg Date: 2014-11 Impact factor: 3.352
Authors: Nusrat S Shommu; Craig N Jenne; Jaime Blackwood; Ari R Joffe; Dori-Ann Martin; Graham C Thompson; Hans J Vogel Journal: PLoS One Date: 2018-03-12 Impact factor: 3.240
Authors: S Ali Naqvi; Graham C Thompson; Ari R Joffe; Jaime Blackwood; Dori-Ann Martin; Mary Brindle; Herman W Barkema; Craig N Jenne Journal: Mediators Inflamm Date: 2019-02-21 Impact factor: 4.711