Literature DB >> 20085792

Freezing of stool samples improves real-time PCR detection of Entamoeba dispar and Entamoeba histolytica.

Lieselotte Cnops1, Marjan Van Esbroeck.   

Abstract

PCR as a tool for intestinal parasite diagnosis is expanding since differentiation between Entamoeba histolytica and Entamoeba dispar cysts is impossible with microscopy. Since pre-analytical factors influence DNA detection, we evaluated with real-time PCR the influence of storage time and temperature. We demonstrated an improved DNA detection in frozen stool samples. Copyright 2010 Elsevier B.V. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20085792     DOI： 10.1016/j.mimet.2010.01.001

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  3 in total

Review 1.  Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.

Authors:  Jaco J Verweij; C Rune Stensvold
Journal:  Clin Microbiol Rev       Date:  2014-04       Impact factor: 26.132

2.  A Schistosoma haematobium-specific real-time PCR for diagnosis of urogenital schistosomiasis in serum samples of international travelers and migrants.

Authors:  Lieselotte Cnops; Patrick Soentjens; Jan Clerinx; Marjan Van Esbroeck
Journal:  PLoS Negl Trop Dis       Date:  2013-08-29

3.  Clinical and microscopic predictors of Entamoeba histolytica intestinal infection in travelers and migrants diagnosed with Entamoeba histolytica/dispar infection.

Authors:  Steven Van Den Broucke; Jacob Verschueren; Marjan Van Esbroeck; Emmanuel Bottieau; Jef Van den Ende
Journal:  PLoS Negl Trop Dis       Date:  2018-10-29
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.