CONCLUSIONS: Results of the current study revealed improved phonation threshold pressure (PTP), normalized mucosal wave amplitude (NMWA), and less contraction of the lamina propria in injured larynges treated with basic fibroblast growth factor (bFGF). OBJECTIVES: We investigated the effects of local injection of bFGF for treatment of acute vocal fold injury in a canine model. METHODS: Vocal folds of eight beagles were unilaterally injured by removal of the mucosa under direct laryngoscopy. Four beagles received local injections of bFGF delivered to the scarred vocal fold at 1 month after injury. The remaining four beagles received local injections of saline and served as a sham-treatment group. Larynges were harvested 5 months after treatment and excised larynx experiments were performed to measure PTP, NMWA, and normalized glottal gap (NGG). Histologic staining was performed to evaluate structural changes of the extracellular matrix. RESULTS: Excised larynx measurements revealed significantly lower PTP and increased NMWA in bFGF-treated vocal fold. Elastica Van Gieson staining revealed less contraction of the bFGF-treated vocal fold. Histologic measurements revealed that the thickness of the lamina propria was significantly greater in the bFGF-treated vocal fold. Alcian blue staining revealed improved restoration of hyaluronic acid in the bFGF-treated vocal fold.
CONCLUSIONS: Results of the current study revealed improved phonation threshold pressure (PTP), normalized mucosal wave amplitude (NMWA), and less contraction of the lamina propria in injured larynges treated with basic fibroblast growth factor (bFGF). OBJECTIVES: We investigated the effects of local injection of bFGF for treatment of acute vocal fold injury in a canine model. METHODS: Vocal folds of eight beagles were unilaterally injured by removal of the mucosa under direct laryngoscopy. Four beagles received local injections of bFGF delivered to the scarred vocal fold at 1 month after injury. The remaining four beagles received local injections of saline and served as a sham-treatment group. Larynges were harvested 5 months after treatment and excised larynx experiments were performed to measure PTP, NMWA, and normalized glottal gap (NGG). Histologic staining was performed to evaluate structural changes of the extracellular matrix. RESULTS: Excised larynx measurements revealed significantly lower PTP and increased NMWA in bFGF-treated vocal fold. Elastica Van Gieson staining revealed less contraction of the bFGF-treated vocal fold. Histologic measurements revealed that the thickness of the lamina propria was significantly greater in the bFGF-treated vocal fold. Alcian blue staining revealed improved restoration of hyaluronic acid in the bFGF-treated vocal fold.
Authors: Joo Hyun Woo; Suzanne N King; Henry Hoffman; Seth Dailey; Sarah Wang; Michael B Christensen; Susan L Thibeault Journal: Otolaryngol Head Neck Surg Date: 2016-05-10 Impact factor: 3.497
Authors: M Graupp; S Bachna-Rotter; C Gerstenberger; G Friedrich; E Fröhlich-Sorger; K Kiesler; M Gugatschka Journal: Eur Arch Otorhinolaryngol Date: 2015-06-25 Impact factor: 2.503
Authors: Josh D Erndt-Marino; Andrea C Jimenez-Vergara; Patricia Diaz-Rodriguez; Jonathan Kulwatno; Juan Felipe Diaz-Quiroz; Susan Thibeault; Mariah S Hahn Journal: J Biomed Mater Res B Appl Biomater Date: 2017-06-05 Impact factor: 3.368
Authors: Hongyu Chen; Josh Erndt-Marino; Patricia Diaz-Rodriguez; Jonathan Kulwatno; Andrea C Jimenez-Vergara; Susan L Thibeault; Mariah S Hahn Journal: J Biomed Mater Res B Appl Biomater Date: 2018-09-05 Impact factor: 3.368