M E García1, C A Marra, O R Rebolledo. 1. Centro de Endocrinología Experimental y Aplicada, UNLP-CONICET La Plata, PAHO/WHO Collaborating Center, Argentina.
Abstract
OBJECTIVE: To study alterations in plasma lipid profile and oxidative damage to lipoprotein fractions (LF) and their fatty acids during an early insulin-resistant and increased oxidative state developed by a fructose-rich diet (FRD). METHODS AND RESULTS: Wistar rats were fed a commercial diet with (FRD) or without (CD) 10% fructose in the drinking water. After 3 weeks, plasma glucose, triglyceride (TG), insulin (I), fructosamine (F), free fatty acids (FFA) and lipid profile (total cholesterol [TC] and HDL-C, LDL-C and VLDL-C sub-fractions) were determined. The insulin sensitivity HOMA index was assessed. FRD-fed rats had higher plasma TG, I, and F levels; increased HOMA; decreased HDL-C and LDL-C; augmented VLDL-C and TC/HDL-C, and TG/HDL-C atherogenic risk scores. LF of FRD rats had increased oxidative damage on the fatty acyl profile and in copper-induced lipoperoxidation. CONCLUSIONS: Fructose feeding early increases the atherogenic risk inducing an insulin resistant-glycoxidative state that affects plasma lipid profiles.
OBJECTIVE: To study alterations in plasma lipid profile and oxidative damage to lipoprotein fractions (LF) and their fatty acids during an early insulin-resistant and increased oxidative state developed by a fructose-rich diet (FRD). METHODS AND RESULTS:Wistar rats were fed a commercial diet with (FRD) or without (CD) 10% fructose in the drinking water. After 3 weeks, plasma glucose, triglyceride (TG), insulin (I), fructosamine (F), free fatty acids (FFA) and lipid profile (total cholesterol [TC] and HDL-C, LDL-C and VLDL-C sub-fractions) were determined. The insulin sensitivity HOMA index was assessed. FRD-fed rats had higher plasma TG, I, and F levels; increased HOMA; decreased HDL-C and LDL-C; augmented VLDL-C and TC/HDL-C, and TG/HDL-C atherogenic risk scores. LF of FRD rats had increased oxidative damage on the fatty acyl profile and in copper-induced lipoperoxidation. CONCLUSIONS:Fructose feeding early increases the atherogenic risk inducing an insulin resistant-glycoxidative state that affects plasma lipid profiles.
Authors: Alejandro Rebolledo; Oscar R Rebolledo; Carlos A Marra; María E García; Ana R Roldán Palomo; Laura Rimorini; Juan J Gagliardino Journal: Cardiovasc Diabetol Date: 2010-10-21 Impact factor: 9.951
Authors: Ran Jin; Jean A Welsh; Ngoc-Anh Le; Jeffrey Holzberg; Puneet Sharma; Diego R Martin; Miriam B Vos Journal: Nutrients Date: 2014-08-08 Impact factor: 5.717