Literature DB >> 20079768

A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil.

Stephan Schulz1, Alfredo Peréz-de-Mora, Marion Engel, Jean Charles Munch, Michael Schloter.   

Abstract

The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content. Copyright 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID: 20079768     DOI: 10.1016/j.mimet.2010.01.005

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  4 in total

1.  Abundance and diversity of n-alkane-degrading bacteria in a forest soil co-contaminated with hydrocarbons and metals: a molecular study on alkB homologous genes.

Authors:  Alfredo Pérez-de-Mora; Marion Engel; Michael Schloter
Journal:  Microb Ecol       Date:  2011-05-13       Impact factor: 4.552

2.  Microbial diversity and abundance in the Xinjiang Luliang long-term water-flooding petroleum reservoir.

Authors:  Peike Gao; Huimei Tian; Guoqiang Li; Hongwen Sun; Ting Ma
Journal:  Microbiologyopen       Date:  2015-02-02       Impact factor: 3.139

3.  An Exogenous Surfactant-Producing Bacillus subtilis Facilitates Indigenous Microbial Enhanced Oil Recovery.

Authors:  Peike Gao; Guoqiang Li; Yanshu Li; Yan Li; Huimei Tian; Yansen Wang; Jiefang Zhou; Ting Ma
Journal:  Front Microbiol       Date:  2016-02-18       Impact factor: 5.640

4.  Representation of dormant and active microbial dynamics for ecosystem modeling.

Authors:  Gangsheng Wang; Melanie A Mayes; Lianhong Gu; Christopher W Schadt
Journal:  PLoS One       Date:  2014-02-18       Impact factor: 3.240

  4 in total

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