| Literature DB >> 20072925 |
Jun Takebayashi1, Jianbin Chen, Akihiro Tai.
Abstract
There are many in vitro methods for evaluating antioxidant activity. In this chapter, we describe an operationally simple cell-based assay, oxidative hemolysis inhibition assay (OxHLIA). OxHLIA is based on inhibition of free radical-induced membrane damage in erythrocytes by antioxidants. The advantage of this method is that it uses peroxyl radicals as pro-oxidants and erythrocytes as oxidizable targets so that the results obtained reflect biologically relevant radical-scavenging activity and microlocalization of antioxidants. We also present here a comparison of OxHLIA with other common methods (DPPH, ABTS(*+), and ORAC assays).Entities:
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Year: 2010 PMID： 20072925 DOI： 10.1007/978-1-60761-411-1_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745