OBJECTIVES: The objectives of this study were to assess the microvessel density (MVD) of intra-sinus grafts after 6 months of wound healing and to study the relationship between revascularization processes and patient clinical variables and habits. MATERIAL AND METHODS: We performed 45 maxillary sinus augmentations with different implant placements in 25 consecutive patients, obtaining bone cores of the grafted area for histological, histomorphometric and immunohistochemical study. Biopsies were also taken from pristine bone in the posterior maxilla (control). RESULTS: All implants survived at 24 months. Biopsies of sinus augmentation areas showed significantly greater remodeling activity vs. pristine bone, with significantly more osteoid lines. The morphometry study revealed 34.88+/-15.2% vital bone, 32.02+/-15.1% non-mineralized tissue and 33.08+/-25.4% remnant anorganic bovine bone particles. The number of CD34-positive vessels was 86.28+/-55.52/mm(2) in graft tissue vs. 31.52+/-13.69/mm(2) in native tissue (P=0.002, Mann-Whitney U=46). The larger amount of non-mineralized tissue in grafts was directly correlated with a higher MVD (r=0.482, P=0.0001, Pearson's test). MVD was affected by the presence of periodontitis or tobacco and alcohol consumption. CONCLUSION: The angiogenesis and revascularization obtained by this type of graft achieve adequate tissue remodeling for osseointegration and are influenced by periodontal disease and tobacco or alcohol consumption.
OBJECTIVES: The objectives of this study were to assess the microvessel density (MVD) of intra-sinus grafts after 6 months of wound healing and to study the relationship between revascularization processes and patient clinical variables and habits. MATERIAL AND METHODS: We performed 45 maxillary sinus augmentations with different implant placements in 25 consecutive patients, obtaining bone cores of the grafted area for histological, histomorphometric and immunohistochemical study. Biopsies were also taken from pristine bone in the posterior maxilla (control). RESULTS: All implants survived at 24 months. Biopsies of sinus augmentation areas showed significantly greater remodeling activity vs. pristine bone, with significantly more osteoid lines. The morphometry study revealed 34.88+/-15.2% vital bone, 32.02+/-15.1% non-mineralized tissue and 33.08+/-25.4% remnant anorganic bovine bone particles. The number of CD34-positive vessels was 86.28+/-55.52/mm(2) in graft tissue vs. 31.52+/-13.69/mm(2) in native tissue (P=0.002, Mann-Whitney U=46). The larger amount of non-mineralized tissue in grafts was directly correlated with a higher MVD (r=0.482, P=0.0001, Pearson's test). MVD was affected by the presence of periodontitis or tobacco and alcohol consumption. CONCLUSION: The angiogenesis and revascularization obtained by this type of graft achieve adequate tissue remodeling for osseointegration and are influenced by periodontal disease and tobacco or alcohol consumption.
Authors: James Carlos Nery; Luís Antônio Violin Dias Pereira; George Furtado Guimarães; Cassio Rocha Scardueli; Fabiana Mantovani Gomes França; Rubens Spin-Neto; Andreas Stavropoulos Journal: Int J Implant Dent Date: 2017-05-04