OBJECTIVE: To investigate the associations between Coombs' testing, haemoplasma and retroviral infections, and feline anaemia. METHODS: Haematology, Coombs' testing (including assessment of persistent autoagglutination) and selected infection testing (haemoplasma, feline leukaemia virus/feline immunodeficiency virus provirus) were performed in blood samples collected from 60 anaemic and 60 non-anaemic cats. RESULTS: No association between infection and anaemia or Coombs' positivity existed. Anaemic cats (21.7%) were significantly more likely than non-anaemic cats (0%) to have cold autoagglutination (P<0.0001), but significance (set at <or=0.0025 due to multiple testing) was not quite reached when Coombs' positivity was compared between anaemic (40.4% and 21.7% positive at 4 degrees C and 37 degrees C, respectively) and non-anaemic (20% and 3.3% positive, P=0.021 and P=0.004, at 4 degrees C and 37 degrees C, respectively) cats. Cats with immune-mediated haemolytic anaemia were significantly more likely to have persistent cold autoagglutination (P<0.0001) and be Coombs' positive at 37 degrees C with polyvalent (P<0.0001), immunoglobulin (Ig)G (P<0.0001) or any antiserum (P<0.0001). Haemoplasmas and retroviruses were uncommonly detected. CLINICAL SIGNIFICANCE: Cats suspected of having immune-mediated haemolytic anaemia should be evaluated for persistent autoagglutination at 4 degrees C as well as performing Coombs' testing at 37 degrees C, but positive results may occur in with other forms of anaemia. Testing for erythrocyte-bound antibodies should always be interpreted in parallel with documentation of haemolysis in anaemic cats.
OBJECTIVE: To investigate the associations between Coombs' testing, haemoplasma and retroviral infections, and feline anaemia. METHODS: Haematology, Coombs' testing (including assessment of persistent autoagglutination) and selected infection testing (haemoplasma, feline leukaemia virus/feline immunodeficiency virus provirus) were performed in blood samples collected from 60 anaemic and 60 non-anaemic cats. RESULTS: No association between infection and anaemia or Coombs' positivity existed. Anaemic cats (21.7%) were significantly more likely than non-anaemic cats (0%) to have cold autoagglutination (P<0.0001), but significance (set at <or=0.0025 due to multiple testing) was not quite reached when Coombs' positivity was compared between anaemic (40.4% and 21.7% positive at 4 degrees C and 37 degrees C, respectively) and non-anaemic (20% and 3.3% positive, P=0.021 and P=0.004, at 4 degrees C and 37 degrees C, respectively) cats. Cats with immune-mediated haemolytic anaemia were significantly more likely to have persistent cold autoagglutination (P<0.0001) and be Coombs' positive at 37 degrees C with polyvalent (P<0.0001), immunoglobulin (Ig)G (P<0.0001) or any antiserum (P<0.0001). Haemoplasmas and retroviruses were uncommonly detected. CLINICAL SIGNIFICANCE: Cats suspected of having immune-mediated haemolytic anaemia should be evaluated for persistent autoagglutination at 4 degrees C as well as performing Coombs' testing at 37 degrees C, but positive results may occur in with other forms of anaemia. Testing for erythrocyte-bound antibodies should always be interpreted in parallel with documentation of haemolysis in anaemic cats.
Authors: Oliver A Garden; Linda Kidd; Angela M Mexas; Yu-Mei Chang; Unity Jeffery; Shauna L Blois; Jonathan E Fogle; Amy L MacNeill; George Lubas; Adam Birkenheuer; Simona Buoncompagni; Julien R S Dandrieux; Antonio Di Loria; Claire L Fellman; Barbara Glanemann; Robert Goggs; Jennifer L Granick; Dana N LeVine; Claire R Sharp; Saralyn Smith-Carr; James W Swann; Balazs Szladovits Journal: J Vet Intern Med Date: 2019-02-26 Impact factor: 3.333
Authors: Armelle deLaforcade; Lenore Bacek; Marie-Claude Blais; Corrin Boyd; Benjamin M Brainard; Daniel L Chan; Stefano Cortellini; Robert Goggs; Guillaume L Hoareau; Amy Koenigshof; Ron Li; Alex Lynch; Alan Ralph; Elizabeth Rozanski; Claire R Sharp Journal: J Vet Emerg Crit Care (San Antonio) Date: 2022-05-02