| Literature DB >> 20067256 |
Yuan He1, Matthew S Macauley, Keith A Stubbs, David J Vocadlo, Gideon J Davies.
Abstract
N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response, and transcriptional regulation. Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) using a substrate-assisted catalytic mechanism. Here we define the reaction coordinate using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.Entities:
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Year: 2010 PMID： 20067256 DOI： 10.1021/ja9086769
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419