Literature DB >> 20066242

Counting single molecules in sub-nanolitre droplets.

T D Rane1, C M Puleo, K J Liu, Y Zhang, A P Lee, T H Wang.   

Abstract

We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction.

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Year:  2009        PMID： 20066242      PMCID： PMC3000353          DOI： 10.1039/b917503b

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  22 in total

1.  A single-molecule method for the quantitation of microRNA gene expression.

Authors:  Lori A Neely; Sonal Patel; Joanne Garver; Michael Gallo; Maria Hackett; Stephen McLaughlin; Mark Nadel; John Harris; Steve Gullans; Jenny Rooke
Journal:  Nat Methods       Date:  2006-01       Impact factor: 28.547

Review 2.  Reactions in droplets in microfluidic channels.

Authors:  Helen Song; Delai L Chen; Rustem F Ismagilov
Journal:  Angew Chem Int Ed Engl       Date:  2006-11-13       Impact factor: 15.336

3.  On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets.

Authors:  N Reginald Beer; Elizabeth K Wheeler; Lorenna Lee-Houghton; Nicholas Watkins; Shanavaz Nasarabadi; Nicole Hebert; Patrick Leung; Don W Arnold; Christopher G Bailey; Bill W Colston
Journal:  Anal Chem       Date:  2008-02-16       Impact factor: 6.986

4.  Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets.

Authors:  Yolanda Schaerli; Robert C Wootton; Tom Robinson; Viktor Stein; Christopher Dunsby; Mark A A Neil; Paul M W French; Andrew J Demello; Chris Abell; Florian Hollfelder
Journal:  Anal Chem       Date:  2009-01-01       Impact factor: 6.986

5.  Dropspots: a picoliter array in a microfluidic device.

Authors:  Christian H J Schmitz; Amy C Rowat; Sarah Köster; David A Weitz
Journal:  Lab Chip       Date:  2008-10-28       Impact factor: 6.799

6.  Single-molecule detection of specific nucleic acid sequences in unamplified genomic DNA.

Authors:  A Castro; J G Williams
Journal:  Anal Chem       Date:  1997-10-01       Impact factor: 6.986

7.  Microfluidic means of achieving attomolar detection limits with molecular beacon probes.

Authors:  Christopher M Puleo; Tza-Huei Wang
Journal:  Lab Chip       Date:  2009-03-06       Impact factor: 6.799

8.  Chemistry and biology in femtoliter and picoliter volume droplets.

Authors:  Daniel T Chiu; Robert M Lorenz
Journal:  Acc Chem Res       Date:  2009-05-19       Impact factor: 22.384

9.  High-throughput single copy DNA amplification and cell analysis in engineered nanoliter droplets.

Authors:  Palani Kumaresan; Chaoyong James Yang; Samantha A Cronier; Robert G Blazej; Richard A Mathies
Journal:  Anal Chem       Date:  2008-04-15       Impact factor: 6.986

10.  Homogeneous point mutation detection by quantum dot-mediated two-color fluorescence coincidence analysis.

Authors:  Hsin-Chih Yeh; Yi-Ping Ho; Ie-Ming Shih; Tza-Huei Wang
Journal:  Nucleic Acids Res       Date:  2006-03-03       Impact factor: 16.971
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  16 in total

1.  Electrothermal Fluid Manipulation of High-Conductivity Samples for Laboratory Automation Applications.

Authors:  Mandy L Y Sin; Vincent Gau; Joseph C Liao; Pak Kin Wong
Journal:  JALA Charlottesv Va       Date:  2010-12-31

Review 2.  Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.

Authors:  Aniruddha M Kaushik; Kuangwen Hsieh; Tza-Huei Wang
Journal:  Wiley Interdiscip Rev Nanomed Nanobiotechnol       Date:  2018-05-24

3.  Microfluidic platform for on-demand generation of spatially indexed combinatorial droplets.

Authors:  Helena Zec; Tushar D Rane; Tza-Huei Wang
Journal:  Lab Chip       Date:  2012-07-19       Impact factor: 6.799

4.  Miniaturized, multiplexed readout of droplet-based microfluidic assays using time-domain modulation.

Authors:  Melaku Muluneh; Bawul Kim; Gershon Buchsbaum; David Issadore
Journal:  Lab Chip       Date:  2014-10-14       Impact factor: 6.799

Review 5.  Advances in microfluidic PCR for point-of-care infectious disease diagnostics.

Authors:  Seungkyung Park; Yi Zhang; Shin Lin; Tza-Huei Wang; Samuel Yang
Journal:  Biotechnol Adv       Date:  2011-06-30       Impact factor: 14.227

6.  A droplet-based, optofluidic device for high-throughput, quantitative bioanalysis.

Authors:  Feng Guo; Michael Ian Lapsley; Ahmad Ahsan Nawaz; Yanhui Zhao; Sz-Chin Steven Lin; Yuchao Chen; Shikuan Yang; Xing-Zhong Zhao; Tony Jun Huang
Journal:  Anal Chem       Date:  2012-11-27       Impact factor: 6.986

7.  Droplet microfluidics for amplification-free genetic detection of single cells.

Authors:  Tushar D Rane; Helena C Zec; Chris Puleo; Abraham P Lee; Tza-Huei Wang
Journal:  Lab Chip       Date:  2012-07-30       Impact factor: 6.799

8.  A serial sample loading system: interfacing multiwell plates with microfluidic devices.

Authors:  Tushar D Rane; Helena C Zec; Tza-Huei Wang
Journal:  J Lab Autom       Date:  2012-08-10

9.  Lab-on-a-chip technologies for single-molecule studies.

Authors:  Yanhui Zhao; Danqi Chen; Hongjun Yue; Jarrod B French; Joseph Rufo; Stephen J Benkovic; Tony Jun Huang
Journal:  Lab Chip       Date:  2013-05-14       Impact factor: 6.799

Review 10.  Analysis of single nucleic acid molecules in micro- and nano-fluidics.

Authors:  Sarah M Friedrich; Helena C Zec; Tza-Huei Wang
Journal:  Lab Chip       Date:  2016-03-07       Impact factor: 6.799
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