Hemvala Chirdchupunseree1, Pornpen Pramyothin. 1. Pharmacological Action of Natural Products Research Unit, Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhyaThai Road, Pathumwan, Bangkok 10330, Thailand.
Abstract
AIM OF THE STUDY: To investigate the protective effect of phyllanthin (a known principal constituent of Phyllanthus amarus Schum. et Thonn.) on ethanol-induced rat liver cell injury. MATERIALS AND METHODS: Primary culture of rat hepatocytes (24h culturing) were pretreated with phyllanthin (1, 2, 3 and 4 microg/ml) for 24h. After 24h pretreatment, cells were treated with ethanol (80 microl/ml) for 2h. RESULTS: Ethanol decreased %MTT, increased the release of transaminases (ALT and AST) with the increase in the production of intracellular ROS and lipid peroxidation. Phyllanthin demonstrated its role in protection by antagonizing the above effect induced by ethanol. Phyllanthin also restored the antioxidant capability of rat hepatocytes including level of total glutathione, and activities of superoxide dismutase (SOD) and glutathione reductase (GR) which were reduced by ethanol. CONCLUSIONS: These results suggested the hepatoprotective effect of phyllanthin against ethanol-induced oxidative stress causing rat liver cell damage through its antioxidant activity. Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.
AIM OF THE STUDY: To investigate the protective effect of phyllanthin (a known principal constituent of Phyllanthus amarus Schum. et Thonn.) on ethanol-induced ratliver cell injury. MATERIALS AND METHODS: Primary culture of rat hepatocytes (24h culturing) were pretreated with phyllanthin (1, 2, 3 and 4 microg/ml) for 24h. After 24h pretreatment, cells were treated with ethanol (80 microl/ml) for 2h. RESULTS:Ethanol decreased %MTT, increased the release of transaminases (ALT and AST) with the increase in the production of intracellular ROS and lipid peroxidation. Phyllanthin demonstrated its role in protection by antagonizing the above effect induced by ethanol. Phyllanthin also restored the antioxidant capability of rat hepatocytes including level of total glutathione, and activities of superoxide dismutase (SOD) and glutathione reductase (GR) which were reduced by ethanol. CONCLUSIONS: These results suggested the hepatoprotective effect of phyllanthin against ethanol-induced oxidative stress causing rat liver cell damage through its antioxidant activity. Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.