Quantification and comparison of ammonia-oxidizing bacterial communities in MBRs treating various types of wastewater.

The community structure and abundance of ammonia-oxidizing bacteria (AOB) in membrane bioreactors (MBRs) constructed to treat different types of wastewater were analyzed by PCR-DGGE, cloning of 16S rRNA fragments and quantitative real-time PCR techniques. DGGE analysis revealed that the diversity of the ammonia-oxidizing bacterial communities was similar among the four reactors. Additionally, variation in influents had no significant effect on the distribution of the AOB communities. Sequencing analysis of screened clones revealed a wide variety of nitrifying organisms and denitrifying bacteria, which suggests that there may be multiple modes of nitrification and denitrification in MBRs. Real-time PCR revealed that the AOB comprised less than 0.1% of the total bacteria in all MBRs. The free ammonia content of the influent was well correlated with the AOB cell numbers. Taken together, these data suggest that higher NH(4)(+)-N content favors the growth of ammonia oxidizers in MBRs. Crown Copyright 2009. Published by Elsevier Ltd. All rights reserved.