Literature DB >> 20059155

A more efficient device for preparing model-membrane liposomes by the rapid solvent exchange method.

Jeffrey T Buboltz1.   

Abstract

We modified the original design for a rapid solvent exchange (RSE) device with the intent of making the RSE method (i) more efficient and (ii) easier to adopt and implement. Our modifications improved solvent-removal kinetics by a factor of 2, while reducing sample-prep time by a factor of 3. In this paper, we develop the kinetic model that informed the device revision and we address several RSE parameters that have not yet been discussed in the literature. We also provide detailed mechanical drawings and present solvent-removal efficiency data that confirm the improved performance of our device.

Entities:  

Year:  2009        PMID： 20059155     DOI： 10.1063/1.3264073

Source DB:  PubMed          Journal:  Rev Sci Instrum        ISSN： 0034-6748            Impact factor:   1.523


  21 in total

1.  Properties of membranes derived from the total lipids extracted from the human lens cortex and nucleus.

Authors:  Laxman Mainali; Marija Raguz; William J O'Brien; Witold K Subczynski
Journal:  Biochim Biophys Acta       Date:  2013-02-21

2.  Formation of cholesterol Bilayer Domains Precedes Formation of Cholesterol Crystals in Membranes Made of the Major Phospholipids of Human Eye Lens Fiber Cell Plasma Membranes.

Authors:  Laxman Mainali; Marta Pasenkiewicz-Gierula; Witold K Subczynski
Journal:  Curr Eye Res       Date:  2019-09-03       Impact factor: 2.424

3.  Effects of Membrane Charge and Order on Membrane Binding of the Retroviral Structural Protein Gag.

Authors:  Yi Wen; Robert A Dick; Gerald W Feigenson; Volker M Vogt
Journal:  J Virol       Date:  2016-09-29       Impact factor: 5.103

4.  Changes in the Properties and Organization of Human Lens Lipid Membranes Occurring with Age.

Authors:  Laxman Mainali; Marija Raguz; William J O'Brien; Witold K Subczynski
Journal:  Curr Eye Res       Date:  2016-10-28       Impact factor: 2.424

5.  Formation of cholesterol bilayer domains precedes formation of cholesterol crystals in cholesterol/dimyristoylphosphatidylcholine membranes: EPR and DSC studies.

Authors:  Laxman Mainali; Marija Raguz; Witold K Subczynski
Journal:  J Phys Chem B       Date:  2013-07-18       Impact factor: 2.991

6.  Using spin-label electron paramagnetic resonance (EPR) to discriminate and characterize the cholesterol bilayer domain.

Authors:  Marija Raguz; Laxman Mainali; Justyna Widomska; Witold K Subczynski
Journal:  Chem Phys Lipids       Date:  2011-08-09       Impact factor: 3.329

7.  Phase-separation and domain-formation in cholesterol-sphingomyelin mixture: pulse-EPR oxygen probing.

Authors:  Laxman Mainali; Marija Raguz; Witold K Subczynski
Journal:  Biophys J       Date:  2011-08-17       Impact factor: 4.033

8.  Spin-label W-band EPR with seven-loop-six-gap resonator: Application to lens membranes derived from eyes of a single donor.

Authors:  Laxman Mainali; Jason W Sidabras; Theodore G Camenisch; Joseph J Ratke; Marija Raguz; James S Hyde; Witold K Subczynski
Journal:  Appl Magn Reson       Date:  2014-12       Impact factor: 0.831

9.  Properties of membranes derived from the total lipids extracted from clear and cataractous lenses of 61-70-year-old human donors.

Authors:  Laxman Mainali; Marija Raguz; William J O'Brien; Witold K Subczynski
Journal:  Eur Biophys J       Date:  2014-12-14       Impact factor: 1.733

10.  Interaction of Alpha-Crystallin with Phospholipid Membranes.

Authors:  Laxman Mainali; William J O'Brien; Raju Timsina
Journal:  Curr Eye Res       Date:  2020-07-12       Impact factor: 2.424
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