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Literature DB >> 20058182

Glycosylated quantum dots for the selective labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains.

Joël Coulon¹, Ilan Thouvenin, Fadi Aldeek, Lavinia Balan, Raphaël Schneider.

Abstract

Highly fluorescent CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting yeast cells. Sugars (mannose, galactose or glucose) were adsorbed on CdTe@TGA QDs and the interaction of these nanoparticles with yeast cells was studied by fluorescence microscopy. Results obtained demonstrate that galactose and mannose functionalized QDs associate respectively with Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains due to saccharide/lectin specific recognition. Glucose-functionalized CdTe QDs, which are not recognized by cell lectins, preferentially localize in the bud scars of S. cerevisiae.

Entities: Chemical Species

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Year: 2010 PMID： 20058182 DOI： 10.1007/s10895-009-0590-8

Source DB: PubMed Journal: J Fluoresc ISSN： 1053-0509 Impact factor: 2.217

34 in total

Glycosylated quantum dots for the selective labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains.

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Review 2. Luminescent quantum dots: a very attractive and promising tool in biomedicine.

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8. Isolation and partial purification of mannose-specific agglutinin from brewer's yeast involved in flocculation.

9. Kluyveromyces bulgaricus yeast lectins. Isolation of two galactose-specific lectin forms from the yeast cell wall.

10. Alternate interactions of the D-galactose-specific yeast lectin Kb-CWL I with sensitive yeast strains.

Review 1. Glyconanomaterials for biosensing applications.

2. Glyconanomaterials: Emerging applications in biomedical research.

Review 3. Semiconductor quantum dots for biomedicial applications.

4. Development and use of a quantum dot probe to track multiple yeast strains in mixed culture.