Literature DB >> 20056038

Simultaneous quantitative determination of cinnamaldehyde and methyl eugenol from stem bark of Cinnamomum zeylanicum Blume using RP-HPLC.

Atish Gursale1, Vidya Dighe, Guarang Parekh.   

Abstract

A simple, sensitive, and precise reversed-phase high performance liquid chromatographic (HPLC) method has been developed, validated and used for simultaneous quantitative determination of cinnamaldehyde and methyl eugenol from the methanolic extract of dried bark powder of Cinnamomum zeylanicum Blume (family Lauraceae). The ultrasonic extraction method was used for the extraction of these compounds. The reversed-phase HPLC analysis was carried out using a Intersil ODS-3V-C(18) (150 mm x 4.6 mm, 5 microm) column and a mobile phase comprising of methanol-acetonitrile-water in the volume ratio of 35:20:45, delivered at a flow rate of 1.0 cm(3)/min. The detection and quantitation of both the compounds was carried out at 221 nm.

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Year:  2010        PMID： 20056038     DOI： 10.1093/chromsci/48.1.59

Source DB:  PubMed          Journal:  J Chromatogr Sci        ISSN： 0021-9665            Impact factor:   1.618


  8 in total

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