Literature DB >> 2005357

Microassay for DNA methyltransferase.

R L Adams1, A Rinaldi, C Seivwright.   

Abstract

A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.

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Year:  1991        PMID： 2005357     DOI： 10.1016/0165-022x(91)90077-a

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  9 in total

1.  SIRT1 deacetylates the DNA methyltransferase 1 (DNMT1) protein and alters its activities.

Authors:  Lirong Peng; Zhigang Yuan; Hongbo Ling; Kenji Fukasawa; Keith Robertson; Nancy Olashaw; John Koomen; Jiandong Chen; William S Lane; Edward Seto
Journal:  Mol Cell Biol       Date:  2011-09-26       Impact factor: 4.272

2.  Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH 3T3 cells.

Authors:  J Wu; J P Issa; J Herman; D E Bassett; B D Nelkin; S B Baylin
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-01       Impact factor: 11.205

3.  Modulation of DNA methyltransferase profile by methyl donor starvation followed by gamma irradiation.

Authors:  Vipen Batra; Kaushala P Mishra
Journal:  Mol Cell Biochem       Date:  2006-07-20       Impact factor: 3.396

4.  Association of arsenic-induced malignant transformation with DNA hypomethylation and aberrant gene expression.

Authors:  C Q Zhao; M R Young; B A Diwan; T P Coogan; M P Waalkes
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-30       Impact factor: 11.205

5.  DNA methylation regulates p27kip1 expression in rodent pituitary cell lines.

Authors:  X Qian; L Jin; E Kulig; R V Lloyd
Journal:  Am J Pathol       Date:  1998-11       Impact factor: 4.307

6.  In vivo stabilization of the Dnmt1 (cytosine-5)- methyltransferase protein.

Authors:  Feng Ding; J Richard Chaillet
Journal:  Proc Natl Acad Sci U S A       Date:  2002-10-23       Impact factor: 11.205

7.  Increased cytosine DNA-methyltransferase activity is target-cell-specific and an early event in lung cancer.

Authors:  S A Belinsky; K J Nikula; S B Baylin; J P Issa
Journal:  Proc Natl Acad Sci U S A       Date:  1996-04-30       Impact factor: 11.205

8.  Methylation-dependent gene silencing induced by interleukin 1beta via nitric oxide production.

Authors:  A Hmadcha; F J Bedoya; F Sobrino; E Pintado
Journal:  J Exp Med       Date:  1999-12-06       Impact factor: 14.307

Review 9.  DNA Methyltransferase Activity Assays: Advances and Challenges.

Authors:  Wan Jun Poh; Cayden Pang Pee Wee; Zhiqiang Gao
Journal:  Theranostics       Date:  2016-01-06       Impact factor: 11.556
  9 in total

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