| Literature DB >> 20052672 |
Peng Huang1, Li Min Lin, Xiao Ying Wu, Qiu Ling Tang, Xue Yong Feng, Guang Yu Lin, Xiaobo Lin, Hong Wu Wang, Tian Hua Huang, Lian Ma.
Abstract
Recent studies have demonstrated that mesenchymal stem cells could differentiate into germ cells under appropriate conditions. We sought to determine whether human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) could form germ cells in vitro. HUMSCs were induced to differentiate into germ cells in all-trans retinoic acid, testosterone and testicular-cell-conditioned medium prepared from newborn male mouse testes. HUMSCs formed "tadpole-like" cells after induction with different reagents and showed both mRNA and protein expression of germ-cell-specific markers Oct4 (POUF5), Ckit, CD49(f) (alpha6), Stella (DDPA3), and Vasa (DDX4). Our results may provide a new route for reproductive therapy involving HUMSCs and a novel in vitro model to investigate the molecular mechanisms that regulate the development of the mammalian germ lineage. (c) 2010 Wiley-Liss, Inc.Entities:
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Year: 2010 PMID： 20052672 DOI： 10.1002/jcb.22453
Source DB: PubMed Journal: J Cell Biochem ISSN： 0730-2312 Impact factor: 4.429