Literature DB >> 20049690

In situ hybridization to identify gut stem cells.

Alex Gregorieff1, Hans Clevers.   

Abstract

In recent years, considerable effort has been directed towards identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.

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Year:  2010        PMID： 20049690     DOI： 10.1002/9780470151808.sc02f01s12

Source DB:  PubMed          Journal:  Curr Protoc Stem Cell Biol        ISSN： 1938-8969


  19 in total

Review 1.  Validating transcripts with probes and imaging technology.

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2.  Colonic healing requires Wnt produced by epithelium as well as Tagln+ and Acta2+ stromal cells.

Authors:  Soumyashree Das; Qiang Feng; Iyshwarya Balasubramanian; Xiang Lin; Haoran Liu; Oscar Pellón-Cardenas; Shiyan Yu; Xiao Zhang; Yue Liu; Zhi Wei; Edward M Bonder; Michael P Verzi; Wei Hsu; Lanjing Zhang; Timothy C Wang; Nan Gao
Journal:  Development       Date:  2022-01-12       Impact factor: 6.868

3.  Fasting Activates Fatty Acid Oxidation to Enhance Intestinal Stem Cell Function during Homeostasis and Aging.

Authors:  Maria M Mihaylova; Chia-Wei Cheng; Amanda Q Cao; Surya Tripathi; Miyeko D Mana; Khristian E Bauer-Rowe; Monther Abu-Remaileh; Laura Clavain; Aysegul Erdemir; Caroline A Lewis; Elizaveta Freinkman; Audrey S Dickey; Albert R La Spada; Yanmei Huang; George W Bell; Vikram Deshpande; Peter Carmeliet; Pekka Katajisto; David M Sabatini; Ömer H Yilmaz
Journal:  Cell Stem Cell       Date:  2018-05-03       Impact factor: 24.633

4.  SPDEF functions as a colorectal tumor suppressor by inhibiting β-catenin activity.

Authors:  Taeko K Noah; Yuan-Hung Lo; Allison Price; Gang Chen; Eileen King; Mary-Kay Washington; Bruce J Aronow; Noah F Shroyer
Journal:  Gastroenterology       Date:  2013-02-01       Impact factor: 22.682

5.  mTOR disruption causes intestinal epithelial cell defects and intestinal atrophy postinjury in mice.

Authors:  Leesa L Sampson; Ashley K Davis; Matthew W Grogg; Yi Zheng
Journal:  FASEB J       Date:  2015-11-30       Impact factor: 5.191

6.  Cdx2 Regulates Intestinal EphrinB1 through the Notch Pathway.

Authors:  Yalun Zhu; Alexa Hryniuk; Tanya Foley; Bradley Hess; David Lohnes
Journal:  Genes (Basel)       Date:  2021-01-28       Impact factor: 4.096

7.  mTORC1 in the Paneth cell niche couples intestinal stem-cell function to calorie intake.

Authors:  Ömer H Yilmaz; Pekka Katajisto; Dudley W Lamming; Yetis Gültekin; Khristian E Bauer-Rowe; Shomit Sengupta; Kivanc Birsoy; Abdulmetin Dursun; V Onur Yilmaz; Martin Selig; G Petur Nielsen; Mari Mino-Kenudson; Lawrence R Zukerberg; Atul K Bhan; Vikram Deshpande; David M Sabatini
Journal:  Nature       Date:  2012-06-28       Impact factor: 49.962

8.  Mouse Nkrp1-Clr gene cluster sequence and expression analyses reveal conservation of tissue-specific MHC-independent immunosurveillance.

Authors:  Qiang Zhang; Mir Munir A Rahim; David S J Allan; Megan M Tu; Simon Belanger; Elias Abou-Samra; Jaehun Ma; Harman S Sekhon; Todd Fairhead; Haggag S Zein; James R Carlyle; Stephen K Anderson; Andrew P Makrigiannis
Journal:  PLoS One       Date:  2012-12-04       Impact factor: 3.240

Review 9.  Quantitative biology of single neurons.

Authors:  James Eberwine; Ditte Lovatt; Peter Buckley; Hannah Dueck; Chantal Francis; Tae Kyung Kim; Jaehee Lee; Miler Lee; Kevin Miyashiro; Jacqueline Morris; Tiina Peritz; Terri Schochet; Jennifer Spaethling; Jai-Yoon Sul; Junhyong Kim
Journal:  J R Soc Interface       Date:  2012-08-22       Impact factor: 4.118

10.  ER stress transcription factor Xbp1 suppresses intestinal tumorigenesis and directs intestinal stem cells.

Authors:  Lukas Niederreiter; Teresa M J Fritz; Timon E Adolph; Anna-Maria Krismer; Felix A Offner; Markus Tschurtschenthaler; Magdalena B Flak; Shuhei Hosomi; Michal F Tomczak; Nicole C Kaneider; Edina Sarcevic; Sarah L Kempster; Tim Raine; Daniela Esser; Philip Rosenstiel; Kenji Kohno; Takao Iwawaki; Herbert Tilg; Richard S Blumberg; Arthur Kaser
Journal:  J Exp Med       Date:  2013-09-16       Impact factor: 14.307
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