Literature DB >> 20049598

Dual fluorochrome flow cytometric assessment of yeast viability.

Bradley Hernlem1, Sui-Sheng Hua.   

Abstract

A novel staining protocol is reported for the assessment of viability in yeast, specifically the biocontrol yeast, Pichia anomala. Employing both the red fluorescent membrane potential sensitive oxonol stain DiBAC(4)(5) (Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol), a structural analog of the commonly used DiBAC(4)(3) (Bis-(1,3-dibutylbarbituric acid)trimethine oxonol), with one of the esterase dependent green fluorogenic probes such as CFDA-AM (5-Carboxyfluorescein diacetate, acetoxymethyl ester) or Calcein-AM (Calcein acetoxymethyl ester), a two-color flow cytometric method was developed, which yields rapid quantitative information on the vitality and vigor of yeast cell cultures. The method was validated by cell sorting and analysis of live, heat killed, and UV-treated yeast.

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Year:  2010        PMID： 20049598     DOI： 10.1007/s00284-009-9576-7

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  15 in total

1.  Membrane potential estimation by flow cytometry.

Authors:  H M Shapiro
Journal:  Methods       Date:  2000-07       Impact factor: 3.608

2.  Survival of the biocontrol yeast Pichia anomala after long-term storage in liquid formulations at different temperatures, assessed by flow cytometry.

Authors:  P Melin; S Håkansson; T H Eberhard; J Schnürer
Journal:  J Appl Microbiol       Date:  2006-02       Impact factor: 3.772

Review 3.  Slow fluorescent indicators of membrane potential: a survey of different approaches to probe response analysis.

Authors:  J Plásek; K Sigler
Journal:  J Photochem Photobiol B       Date:  1996-04       Impact factor: 6.252

4.  Flow cytometry and cell sorting for yeast viability assessment and cell selection.

Authors:  D Deere; J Shen; G Vesey; P Bell; P Bissinger; D Veal
Journal:  Yeast       Date:  1998-01-30       Impact factor: 3.239

5.  Yeast vitality during cider fermentation: assessment by energy metabolism.

Authors:  M G Dinsdale; D Lloyd; P McIntyre; B Jarvis
Journal:  Yeast       Date:  1999-03-15       Impact factor: 3.239

6.  Characterization of the steady-state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Dibac4(3)) in model systems and cells.

Authors:  D E Epps; M L Wolfe; V Groppi
Journal:  Chem Phys Lipids       Date:  1994-02       Impact factor: 3.329

7.  Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and a ratiometric technique.

Authors:  D Novo; N G Perlmutter; R H Hunt; H M Shapiro
Journal:  Cytometry       Date:  1999-01-01

8.  Use of flow cytometry to monitor cell damage and predict fermentation activity of dried yeasts.

Authors:  P V Attfield; S Kletsas; D A Veal; R van Rooijen; P J Bell
Journal:  J Appl Microbiol       Date:  2000-08       Impact factor: 3.772

9.  Interactions of saprophytic yeasts with a nor mutant of Aspergillus flavus.

Authors:  S S Hua; J L Baker; M Flores-Espiritu
Journal:  Appl Environ Microbiol       Date:  1999-06       Impact factor: 4.792

10.  Comparative measurements of membrane potentials with microelectrodes and voltage-sensitive dyes.

Authors:  T Bräuner; D F Hülser; R J Strasser
Journal:  Biochim Biophys Acta       Date:  1984-04-11
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  4 in total

1.  Intracellular trehalose and sorbitol synergistically promoting cell viability of a biocontrol yeast, Pichia anomala, for aflatoxin reduction.

Authors:  Sui Sheng T Hua; Bradley J Hernlem; Wallace Yokoyama; Siov Bouy L Sarreal
Journal:  World J Microbiol Biotechnol       Date:  2015-02-21       Impact factor: 3.312

2.  A method for quantifying sporulation efficiency and isolating meiotic progeny in non-GMO strains of Saccharomyces cerevisiae.

Authors:  Amy Sirr; Martin S Timour; Gareth A Cromie; Michelle Tang; Aimée M Dudley
Journal:  Yeast       Date:  2022-06-23       Impact factor: 3.325

3.  Novel image cytometric method for detection of physiological and metabolic changes in Saccharomyces cerevisiae.

Authors:  Leo L Chan; Alexandria Kury; Alisha Wilkinson; Charlotte Berkes; Alnoor Pirani
Journal:  J Ind Microbiol Biotechnol       Date:  2012-08-05       Impact factor: 3.346

4.  A novel staining protocol for multiparameter assessment of cell heterogeneity in Phormidium populations (cyanobacteria) employing fluorescent dyes.

Authors:  Daria Tashyreva; Josef Elster; Daniela Billi
Journal:  PLoS One       Date:  2013-02-20       Impact factor: 3.240
  4 in total

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