BACKGROUND: Recently, Herfarth et al. reported that a subset of colorectal tumors was characterized by a specific methylation pattern in the MGMT promoter associated with reduced MGMT expression. MATERIALS AND METHODS: Methylation status of the MGMT gene was examined in primary carcinomas and the corresponding normal tissues derived from 38 patients with gastric cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated. RESULTS: Aberrant methylation of the MGMT gene was detected in 4 out of the 38 (11%) primary gastric carcinomas, suggesting that the methylation of MGMT is observed in gastric carcinomas as well as colorectal ones. The clinicopathological data were correlated with the methylation results. A significant difference was observed in the extent of tumor (p=0.0470), lymph node metastasis (p=0.0470), and TNM stage (p=0.0377) (Table I). Moreover, a trend was shown toward large maximal tumor size in methylated tumors (p=0.134). CONCLUSION: MGMT was more frequently methylated in advanced gastric carcinomas.
BACKGROUND: Recently, Herfarth et al. reported that a subset of colorectal tumors was characterized by a specific methylation pattern in the MGMT promoter associated with reduced MGMT expression. MATERIALS AND METHODS: Methylation status of the MGMT gene was examined in primary carcinomas and the corresponding normal tissues derived from 38 patients with gastric cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated. RESULTS: Aberrant methylation of the MGMT gene was detected in 4 out of the 38 (11%) primary gastric carcinomas, suggesting that the methylation of MGMT is observed in gastric carcinomas as well as colorectal ones. The clinicopathological data were correlated with the methylation results. A significant difference was observed in the extent of tumor (p=0.0470), lymph node metastasis (p=0.0470), and TNM stage (p=0.0377) (Table I). Moreover, a trend was shown toward large maximal tumor size in methylated tumors (p=0.134). CONCLUSION:MGMT was more frequently methylated in advanced gastric carcinomas.
Authors: Arif Akbar Bhat; Hilal Ahmad Wani; Ajaz Ahmad Waza; Rawoof Ahmad Malik; Akbar Masood; Showkat Jeelani; Showkat Kadla; Sabhiya Majid Journal: J Gastrointest Oncol Date: 2016-12
Authors: Gary C Hon; R David Hawkins; Otavia L Caballero; Christine Lo; Ryan Lister; Mattia Pelizzola; Armand Valsesia; Zhen Ye; Samantha Kuan; Lee E Edsall; Anamaria Aranha Camargo; Brian J Stevenson; Joseph R Ecker; Vineet Bafna; Robert L Strausberg; Andrew J Simpson; Bing Ren Journal: Genome Res Date: 2011-12-07 Impact factor: 9.043
Authors: Qi Zhao; Ewen F Kirkness; Otavia L Caballero; Pedro A Galante; Raphael B Parmigiani; Lee Edsall; Samantha Kuan; Zhen Ye; Samuel Levy; Ana Tereza R Vasconcelos; Bing Ren; Sandro J de Souza; Anamaria A Camargo; Andrew J G Simpson; Robert L Strausberg Journal: Genome Biol Date: 2010-11-25 Impact factor: 13.583