Study on the inclusion interaction of cucurbit[n]urils with sanguinarine by spectrofluorimetry and its analytical application.

The characteristics of host-guest complexation between cucurbit[n]uril (CB[n], n=5, 6, 7, 8) and sanguinarine (SA) were investigated by spectrofluorimetry. The result showed that CB[n] (n=5, 6, 7, 8) reacted with SA to form an inclusion complex. At the optimum reaction conditions, the rectilinear calibration graphs were obtained in the range from 0.1 to 2800 ng mL(-1) for the investigated drug, with correlation coefficients 0.9998, 0.9997, 0.9999 and 0.9997 and detection limits 0.052, 0.26, 0.03 and 0.058 ng mL(-1) using CB[5], CB[6], CB[7], and CB[8], respectively. The apparent association constants of the complexes were 1.23 x 10(4), 6.73 x 10(3), 4.53 x 10(4) and 1.21 x 10(4) L mol(-1), for the reagents in the same order. The result demonstrated that CB[7] exhibited a higher fluorescence signal than CB[5], CB[6], and CB[8]. The proposed method was successfully applied for the determination of the drug in human urine and serum samples. Copyright (c) 2009 Elsevier B.V. All rights reserved.