OBJECTIVE: Hemodialysis (HD) patients lose lean body mass, even when they are adequately dialysed. One reason may be a decreased activity of the IGF-system. However, data on changes in bioactive IGF-I during HD are sparse. DESIGN: Ten stable, non-diabetic HD patients were studied with 30 min intervals during a scheduled HD, with blood sampling before (-15 and 0 min), during (4 h) and after (1 h) the session. Patients were fasted for at least 6 h before and during the study. Arterial and venous blood was sampled for determination of IGF-I bioactivity, free and total IGF-I and IGF-II, IGF binding protein-1 (IGFBP-1), IGFBP-1 complexed IGF-I and IGFBP-2. RESULTS: Total IGF-I and -II decreased marginally (<12%) at the very end of the study (P<0.05). By contrast, at 3 h free and bioactive IGF-I had declined by approximately 35% and 50%, respectively, and levels remained suppressed for the rest of the study (P<0.05). Concomitantly, IGFBP-1 and IGFBP-1:IGF-I complex formation increased 5.0-fold and 2.6-fold, respectively (P<0.05). By contrast, IGFBP-2 did not increase as a result of HD. No major differences between arterial and venous concentrations were observed. CONCLUSION: Despite marginal reductions in total IGF-I and -II, bioactive and free IGF-I declined markedly during and after HD. This is likely a consequence of the increase in IGFBP-1, sequestering free IGF-I, and reducing bioactive IGF-I. Based on the present data we hypothesize that the catabolism induced by HD is in part related to the observed reductions in bioactive IGF-I. Copyright (c) 2009 Elsevier Ltd. All rights reserved.
OBJECTIVE: Hemodialysis (HD) patients lose lean body mass, even when they are adequately dialysed. One reason may be a decreased activity of the IGF-system. However, data on changes in bioactive IGF-I during HD are sparse. DESIGN: Ten stable, non-diabeticHDpatients were studied with 30 min intervals during a scheduled HD, with blood sampling before (-15 and 0 min), during (4 h) and after (1 h) the session. Patients were fasted for at least 6 h before and during the study. Arterial and venous blood was sampled for determination of IGF-I bioactivity, free and total IGF-I and IGF-II, IGF binding protein-1 (IGFBP-1), IGFBP-1 complexed IGF-I and IGFBP-2. RESULTS: Total IGF-I and -II decreased marginally (<12%) at the very end of the study (P<0.05). By contrast, at 3 h free and bioactive IGF-I had declined by approximately 35% and 50%, respectively, and levels remained suppressed for the rest of the study (P<0.05). Concomitantly, IGFBP-1 and IGFBP-1:IGF-I complex formation increased 5.0-fold and 2.6-fold, respectively (P<0.05). By contrast, IGFBP-2 did not increase as a result of HD. No major differences between arterial and venous concentrations were observed. CONCLUSION: Despite marginal reductions in total IGF-I and -II, bioactive and free IGF-I declined markedly during and after HD. This is likely a consequence of the increase in IGFBP-1, sequestering free IGF-I, and reducing bioactive IGF-I. Based on the present data we hypothesize that the catabolism induced by HD is in part related to the observed reductions in bioactive IGF-I. Copyright (c) 2009 Elsevier Ltd. All rights reserved.
Authors: Zeinab Sarem; Christiane Bumke-Vogt; Ayman M Mahmoud; Biruhalem Assefa; Martin O Weickert; Aikatarini Adamidou; Volker Bähr; Jan Frystyk; Matthias Möhlig; Joachim Spranger; Stefanie Lieske; Andreas L Birkenfeld; Andreas F H Pfeiffer; Ayman M Arafat Journal: J Clin Endocrinol Metab Date: 2017-09-01 Impact factor: 5.958
Authors: Esther Nkuipou-Kenfack; Akshay Bhat; Julie Klein; Vera Jankowski; William Mullen; Antonia Vlahou; Mohammed Dakna; Thomas Koeck; Joost P Schanstra; Petra Zürbig; Karl L Rudolph; Björn Schumacher; Andreas Pich; Harald Mischak Journal: Oncotarget Date: 2015-10-27
Authors: Mark Reinhard; Jan Frystyk; Bente Jespersen; Mette Bjerre; Jens S Christiansen; Allan Flyvbjerg; Per Ivarsen Journal: BMC Nephrol Date: 2013-04-04 Impact factor: 2.388