BACKGROUND: Biofilms play a role in the pathogenesis of a variety of otorhinolaryngologic diseases, including otitis media and cholesteatoma. Despite this, relatively few studies have undertaken to demonstrate the presence of biofilms tissues from patients with chronic otitis media or infected cholesteatoma. OBJECTIVE/HYPOTHESIS: Our objective is to detect evidence of biofilms human chronic ear infections with scanning electron microscopy (SEM). We hypothesized that bacterial biofilms are present in patients with chronic otitis media. STUDY DESIGN: We performed prospective collection of tissue collected during middle ear surgery from 16 patients undergoing middle ear or mastoid surgery with chronic ear infections. METHODS: A total of 31 middle and mastoid tissue samples were harvested at the time of surgery and processed with critical point drying for SEM analysis. Samples were then searched for evidence of biofilms. RESULTS: Bacterial-shaped objects were identified that displayed both surface binding and the presence of a glycocalyx in 4 patients, findings consistent with bacterial biofilms. Most of these (3 of 4) were in patients with infected cholesteatoma, and biofims were identified in 60% of cholesteatoma cases (3 of 5). On the other hand, only 1 of 7 cases with chronic suppurative otitis media had evidence of biofilms. CONCLUSION: SEM supports the hypothesis that bacterial biofilms are common in chronic infections associated with cholesteatoma and are present in some cases of chronic suppurative otitis media without cholesteatoma.
BACKGROUND: Biofilms play a role in the pathogenesis of a variety of otorhinolaryngologic diseases, including otitis media and cholesteatoma. Despite this, relatively few studies have undertaken to demonstrate the presence of biofilms tissues from patients with chronic otitis media or infected cholesteatoma. OBJECTIVE/HYPOTHESIS: Our objective is to detect evidence of biofilms human chronic ear infections with scanning electron microscopy (SEM). We hypothesized that bacterial biofilms are present in patients with chronic otitis media. STUDY DESIGN: We performed prospective collection of tissue collected during middle ear surgery from 16 patients undergoing middle ear or mastoid surgery with chronic ear infections. METHODS: A total of 31 middle and mastoid tissue samples were harvested at the time of surgery and processed with critical point drying for SEM analysis. Samples were then searched for evidence of biofilms. RESULTS: Bacterial-shaped objects were identified that displayed both surface binding and the presence of a glycocalyx in 4 patients, findings consistent with bacterial biofilms. Most of these (3 of 4) were in patients with infected cholesteatoma, and biofims were identified in 60% of cholesteatoma cases (3 of 5). On the other hand, only 1 of 7 cases with chronic suppurative otitis media had evidence of biofilms. CONCLUSION: SEM supports the hypothesis that bacterial biofilms are common in chronic infections associated with cholesteatoma and are present in some cases of chronic suppurative otitis media without cholesteatoma.
Authors: Patricia A Schachern; Geeyoun Kwon; David E Briles; Patricia Ferrieri; Steven Juhn; Sebahattin Cureoglu; Michael M Paparella; Vladimir Tsuprun Journal: JAMA Otolaryngol Head Neck Surg Date: 2017-10-01 Impact factor: 6.223
Authors: Helen Van Hoecke; Ann-Sophie De Paepe; Edward Lambert; Jonas D Van Belleghem; Piet Cools; Leen Van Simaey; Pieter Deschaght; Mario Vaneechoutte; Ingeborg Dhooge Journal: Eur Arch Otorhinolaryngol Date: 2016-03-05 Impact factor: 2.503
Authors: Rahul Mittal; Christopher V Lisi; Robert Gerring; Jeenu Mittal; Kalai Mathee; Giri Narasimhan; Rajeev K Azad; Qi Yao; M'hamed Grati; Denise Yan; Adrien A Eshraghi; Simon I Angeli; Fred F Telischi; Xue-Zhong Liu Journal: J Med Microbiol Date: 2015-08-05 Impact factor: 2.472
Authors: Bin Yang; Cong Tian; Zhi-guang Zhang; Feng-chan Han; Rami Azem; Heping Yu; Ye Zheng; Ge Jin; James E Arnold; Qing Y Zheng Journal: PLoS One Date: 2011-07-27 Impact factor: 3.240
Authors: Anke Leichtle; Christin Klenke; Joerg Ebmeyer; Markus Daerr; Karl-Ludwig Bruchhage; Anna Sophie Hoffmann; Allen F Ryan; Barbara Wollenberg; Holger Sudhoff Journal: Biomed Res Int Date: 2015-04-02 Impact factor: 3.411