BACKGROUND: Urine 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a specific biomarker of oxidative stress. We evaluated a modified LC-MS/MS assay for urine 8-oxodG and determined biological variation in healthy adults. METHOD: Untreated urine was injected into an isocratic LC-MS/MS system (positive-ion MRM mode). Urine 8-oxodG in 51 healthy volunteers was measured; within- and between-day variations in 23 healthy volunteers were investigated. RESULTS: Dose-response was linear to 452 nmol/l; limit of detection=2.3 nmol/l; within-run and between-run CVs were <3.0% and <4.7%, respectively; recovery=97%-101%; accuracy=97.7-103.5%. Urine 8-oxodG (median, mean [SD]): 1.70, 1.70[0.60]nmol/mmol creatinine (n=51). Men had higher (p=0.027) concentrations than women matched for age and body mass index: mean [SD]: 1.90[1.60]; n=26 vs. 1.50[0.55]; n=25. Within- and between-day variations were wide but random. No significant differences were seen overall across time-points within 1 day or at the same time-point across 5 consecutive days. CONCLUSIONS: The method has advantages of speed and relative simplicity as it does not require sample pre-treatment for 8-oxodG extraction, the use of internal standard or gradient LC elution and has high linearity, specificity, precision and recovery. Biological variation in urine 8-oxodG is wide, but no within- or between-day differences at the group concentration were seen in healthy adults. Copyright 2009 Elsevier B.V. All rights reserved.
BACKGROUND: Urine 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a specific biomarker of oxidative stress. We evaluated a modified LC-MS/MS assay for urine 8-oxodG and determined biological variation in healthy adults. METHOD: Untreated urine was injected into an isocratic LC-MS/MS system (positive-ion MRM mode). Urine 8-oxodG in 51 healthy volunteers was measured; within- and between-day variations in 23 healthy volunteers were investigated. RESULTS: Dose-response was linear to 452 nmol/l; limit of detection=2.3 nmol/l; within-run and between-run CVs were <3.0% and <4.7%, respectively; recovery=97%-101%; accuracy=97.7-103.5%. Urine 8-oxodG (median, mean [SD]): 1.70, 1.70[0.60]nmol/mmol creatinine (n=51). Men had higher (p=0.027) concentrations than women matched for age and body mass index: mean [SD]: 1.90[1.60]; n=26 vs. 1.50[0.55]; n=25. Within- and between-day variations were wide but random. No significant differences were seen overall across time-points within 1 day or at the same time-point across 5 consecutive days. CONCLUSIONS: The method has advantages of speed and relative simplicity as it does not require sample pre-treatment for 8-oxodG extraction, the use of internal standard or gradient LC elution and has high linearity, specificity, precision and recovery. Biological variation in urine 8-oxodG is wide, but no within- or between-day differences at the group concentration were seen in healthy adults. Copyright 2009 Elsevier B.V. All rights reserved.
Authors: Eppie M Yiu; Geneieve Tai; Roger E Peverill; Katherine J Lee; Kevin D Croft; Trevor A Mori; Barbara Scheiber-Mojdehkar; Brigitte Sturm; Monika Praschberger; Adam P Vogel; Gary Rance; Sarah E M Stephenson; Joseph P Sarsero; Creina Stockley; Chung-Yung J Lee; Andrew Churchyard; Marguerite V Evans-Galea; Monique M Ryan; Paul J Lockhart; Louise A Corben; Martin B Delatycki Journal: J Neurol Date: 2015-04-07 Impact factor: 4.849
Authors: Darren R Brenner; Dominique Scherer; Kenneth Muir; Joellen Schildkraut; Paolo Boffetta; Margaret R Spitz; Loic Le Marchand; Andrew T Chan; Ellen L Goode; Cornelia M Ulrich; Rayjean J Hung Journal: Cancer Epidemiol Biomarkers Prev Date: 2014-06-24 Impact factor: 4.254
Authors: Patricia M W Lam; Vilas Mistry; Timothy H Marczylo; Justin C Konje; Mark D Evans; Marcus S Cooke Journal: Free Radic Biol Med Date: 2012-04-15 Impact factor: 7.376
Authors: Annette M Krais; Julie Y Essig; Louise Gren; Carolina Vogs; Eva Assarsson; Katrin Dierschke; Jörn Nielsen; Bo Strandberg; Joakim Pagels; Karin Broberg; Christian H Lindh; Anders Gudmundsson; Aneta Wierzbicka Journal: Int J Environ Res Public Health Date: 2021-06-16 Impact factor: 3.390
Authors: Ai-Ching Boon; Alfred K Lam; Vinod Gopalan; Iris F Benzie; David Briskey; Jeff S Coombes; Robert G Fassett; Andrew C Bulmer Journal: Sci Rep Date: 2015-10-26 Impact factor: 4.379