OBJECTIVES: Agrimoniae Herba has been used as an anti-inflammatory agent in traditional medicine. Nitric oxide and proinflammatory cytokines produced by activated microglia may be a possible etiological factor of neurodegenerative disorders. We evaluated whether Agrimoniae Herba could have an anti-inflammatory effect on lipopolysaccharide-induced BV2 microglial cells. METHODS: The effects of Agrimoniae Herba on lipopolysaccharide-induced nitric oxide and proinflammatory cytokine production in BV2 microglial cells were evaluated by nitric oxide assay, enzyme-linked immunosorbent assay and western blotting. RESULTS: Agrimoniae Herba had no cytotoxicity and suppressed lipopolysaccharide-induced nitric oxide production in BV2 microglial cells. Agrimoniae Herba also suppressed lipopolysaccharide-induced production of proinflammatory cytokines such as tumor necrosis factor, interleukin 1 beta and interleukin 6 in a dose-dependent manner. Agrimoniae Herba inhibited the expression of inducible nitric oxide synthase. DISCUSSION: Taken together, these findings indicate that Agrimoniae Herba may be used as a form of pharmaceutical acupuncture therapy in the treatment of brain inflammation.
OBJECTIVES: Agrimoniae Herba has been used as an anti-inflammatory agent in traditional medicine. Nitric oxide and proinflammatory cytokines produced by activated microglia may be a possible etiological factor of neurodegenerative disorders. We evaluated whether Agrimoniae Herba could have an anti-inflammatory effect on lipopolysaccharide-induced BV2 microglial cells. METHODS: The effects of Agrimoniae Herba on lipopolysaccharide-induced nitric oxide and proinflammatory cytokine production in BV2 microglial cells were evaluated by nitric oxide assay, enzyme-linked immunosorbent assay and western blotting. RESULTS: Agrimoniae Herba had no cytotoxicity and suppressed lipopolysaccharide-induced nitric oxide production in BV2 microglial cells. Agrimoniae Herba also suppressed lipopolysaccharide-induced production of proinflammatory cytokines such as tumor necrosis factor, interleukin 1 beta and interleukin 6 in a dose-dependent manner. Agrimoniae Herba inhibited the expression of inducible nitric oxide synthase. DISCUSSION: Taken together, these findings indicate that Agrimoniae Herba may be used as a form of pharmaceutical acupuncture therapy in the treatment of brain inflammation.
Authors: Telmo N Santos; Gustavo Costa; J Pinto Ferreira; Joana Liberal; Vera Francisco; António Paranhos; Maria T Cruz; M Castelo-Branco; I Vitória Figueiredo; M Teresa Batista Journal: Evid Based Complement Alternat Med Date: 2017-04-12 Impact factor: 2.629
Authors: Sylvia Vogl; Paolo Picker; Judit Mihaly-Bison; Nanang Fakhrudin; Atanas G Atanasov; Elke H Heiss; Christoph Wawrosch; Gottfried Reznicek; Verena M Dirsch; Johannes Saukel; Brigitte Kopp Journal: J Ethnopharmacol Date: 2013-06-13 Impact factor: 4.360
Authors: Tobias Gräber; Holger Kluge; Sebastian Granica; Gert Horn; Corinna Brandsch; Gabriele I Stangl Journal: BMC Vet Res Date: 2014-09-09 Impact factor: 2.741