| Literature DB >> 20033631 |
Frank Cichocki1, Jeffrey S Miller.
Abstract
The in vitro culture system outlined in this chapter allows for the delineation of events that occur during the development of CD34(+) hematopoietic precursor cells into mature KIR(+) human NK cells. This system can also be utilized to study the effects of gene overexpression or knockdown on the process of NK cell differentiation through retroviral transduction and long-term culture. The necessary soluble factors and contact-dependent conditions for in vitro human NK cell development have been worked out in our laboratory over the past 16 years.Entities:
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Year: 2010 PMID： 20033631 DOI： 10.1007/978-1-60761-362-6_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745