OBJECTIVE: To assess whether serum levels of CC and CXC chemokines correlate with disease activity in patients with rheumatoid arthritis (RA), and to determine whether these effects predict clinical response. METHODS: Serum levels of the chemokines CC (CCL2, CCL5) and CXC (CXCL8, CXCL9, CXCL10) were quantified at baseline and after 12 weeks of treatment with disease-modifying antirheumatic drugs or biologic agents in 28 patients using flow cytometry. Serum from 40 healthy individuals was collected for comparison at baseline. Response to treatment was classified according to the European League Against Rheumatism (EULAR) response criteria. Remission of disease was defined as a Disease Activity Score < 2.6. RESULTS: The baseline serum concentrations of CC and CXC chemokines were significantly elevated in patients with active RA compared to healthy controls (p < 0.05) except for CCL2. Significant improvement in all disease activity measurements was observed after 12 weeks of treatment. Seventeen (60.7%) patients achieved good to moderate response based on the EULAR response criteria, and 5 (17.9%) patients achieved remission. The improvement in clinical activity in patients with RA was accompanied by a significant reduction in the serum concentration of CXCL9 and CXCL10 (p < 0.001). A significant reduction in the serum level of CXCL10 was also observed in the group that achieved EULAR response. Serum concentration of CCL5 remained significantly elevated in patients with RA (n = 5) who achieved remission compared to the healthy controls (p < 0.05). CONCLUSION: Serum concentration of CXCL9 and CXCL10 may serve as sensitive biomarkers for disease activity in patients with RA.
OBJECTIVE: To assess whether serum levels of CC and CXC chemokines correlate with disease activity in patients with rheumatoid arthritis (RA), and to determine whether these effects predict clinical response. METHODS: Serum levels of the chemokines CC (CCL2, CCL5) and CXC (CXCL8, CXCL9, CXCL10) were quantified at baseline and after 12 weeks of treatment with disease-modifying antirheumatic drugs or biologic agents in 28 patients using flow cytometry. Serum from 40 healthy individuals was collected for comparison at baseline. Response to treatment was classified according to the European League Against Rheumatism (EULAR) response criteria. Remission of disease was defined as a Disease Activity Score < 2.6. RESULTS: The baseline serum concentrations of CC and CXC chemokines were significantly elevated in patients with active RA compared to healthy controls (p < 0.05) except for CCL2. Significant improvement in all disease activity measurements was observed after 12 weeks of treatment. Seventeen (60.7%) patients achieved good to moderate response based on the EULAR response criteria, and 5 (17.9%) patients achieved remission. The improvement in clinical activity in patients with RA was accompanied by a significant reduction in the serum concentration of CXCL9 and CXCL10 (p < 0.001). A significant reduction in the serum level of CXCL10 was also observed in the group that achieved EULAR response. Serum concentration of CCL5 remained significantly elevated in patients with RA (n = 5) who achieved remission compared to the healthy controls (p < 0.05). CONCLUSION: Serum concentration of CXCL9 and CXCL10 may serve as sensitive biomarkers for disease activity in patients with RA.
Authors: Anouk van Hooij; Debbie M Boeters; Elisa M Tjon Kon Fat; Susan J F van den Eeden; Paul L A M Corstjens; Annette H M van der Helm-van Mil; Annemieke Geluk Journal: Clin Vaccine Immunol Date: 2017-08-04
Authors: Daniel Kotrych; Violetta Dziedziejko; Krzysztof Safranow; Marek Drozdzik; Andrzej Pawlik Journal: Rheumatol Int Date: 2015-02-22 Impact factor: 2.631
Authors: Bridgette D Furman; Collin L Kent; Janet L Huebner; Virginia B Kraus; Amy L McNulty; Farshid Guilak; Steven A Olson Journal: J Orthop Res Date: 2017-11-20 Impact factor: 3.494
Authors: S Yoshida; F Arakawa; F Higuchi; Y Ishibashi; M Goto; Y Sugita; Y Nomura; D Niino; K Shimizu; R Aoki; K Hashikawa; Y Kimura; K Yasuda; K Tashiro; S Kuhara; K Nagata; K Ohshima Journal: Scand J Rheumatol Date: 2012-03-09 Impact factor: 3.641
Authors: Alyson A Kelvin; David Banner; Giuliano Silvi; Maria Luisa Moro; Nadir Spataro; Paolo Gaibani; Francesca Cavrini; Anna Pierro; Giada Rossini; Mark J Cameron; Jesus F Bermejo-Martin; Stéphane G Paquette; Luoling Xu; Ali Danesh; Amber Farooqui; Ilaria Borghetto; David J Kelvin; Vittorio Sambri; Salvatore Rubino Journal: PLoS Negl Trop Dis Date: 2011-08-16