Miaorong Pei1, Xiujun Duan, Xiangping Pei. 1. Department of Chinese Materia Medica, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China. peimr602@163.com
Abstract
OBJECTIVE: To determine the change pattern of alkaline and acid components of Dahuang and Huangbai amoung different combinations in Dahuang Xiaoshi decotion. METHOD: The contents of anthraquinones and berberine were determined by HPLC in samples of Dahuang extracts, Huangbai extracts, Dahuang and Huangbai pair medicine extracts, and extracts of whole recipe. RESULT: The contents of anthraquinones and berberine were decreased with the changes of combinations: from component medicinal material alone to pair medicines, and to whole recipe. the change pattern was more apparent in water decoctions than in ethanol extracts. CONCLUSION: The contents of alkaline and acid components are changed with different extract methods and different combinations.
OBJECTIVE: To determine the change pattern of alkaline and acid components of Dahuang and Huangbai amoung different combinations in Dahuang Xiaoshi decotion. METHOD: The contents of anthraquinones and berberine were determined by HPLC in samples of Dahuang extracts, Huangbai extracts, Dahuang and Huangbai pair medicine extracts, and extracts of whole recipe. RESULT: The contents of anthraquinones and berberine were decreased with the changes of combinations: from component medicinal material alone to pair medicines, and to whole recipe. the change pattern was more apparent in water decoctions than in ethanol extracts. CONCLUSION: The contents of alkaline and acid components are changed with different extract methods and different combinations.