Literature DB >> 200268

Purification and characterization of baboon endogenous virus DNA polymerase.

P S Sarin, B Friedman, R C Gallo.   

Abstract

An RNA-directed DNA polymerase was purified from baboon endogenous type-C virus by successive column chromatography on DEAE cellulose, phosphocellulose and hydroxyapatite. The purified DNA polymerase has a molecular weight of 68 000, a pH optimum of 8.0, a Mn2+ optimum of 1 mM, and a KCl optimum of 40 mM. The purified enzyme transcribes heteropolymeric regions of viral 60--70 S RNA isolated from different type-C viruses. The purified enzyme is immunologically related to a similarly purified polymerase from the cat endogenous type-C virus RD114.

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Year:  1977        PMID： 200268     DOI： 10.1016/0005-2787(77)90140-x

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  4 in total

Review 1.  Immunological characterization of reverse transcriptase from human tumor tissues.

Authors:  P Chandra
Journal:  Surv Immunol Res       Date:  1983

2.  Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.

Authors:  P C Zamecnik; J Goodchild; Y Taguchi; P S Sarin
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

3.  Evidence in patients with systemic lupus erythematosus of the presence of antibodies against RNA-dependent DNA polymerase of baboon endogenous virus.

Authors:  T Okamoto; T Tamura; T Takano
Journal:  Clin Exp Immunol       Date:  1983-12       Impact factor: 4.330

4.  High incidence of human type-C retrovirus (HTLV) in family members of a HTLV-positive Japanese T-cell leukemia patient.

Authors:  P S Sarin; T Aoki; A Shibata; Y Ohnishi; Y Aoyagi; H Miyakoshi; I Emura; V S Kalyanaraman; M Robert-Guroff; M Popovic; M Sarngadharan; P C Nowell; R C Gallo
Journal:  Proc Natl Acad Sci U S A       Date:  1983-04       Impact factor: 11.205
  4 in total

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