Literature DB >> 20026205

Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge.

Jeffrey C Lord1, Kris Hartzer, Michelle Toutges, Brenda Oppert.   

Abstract

To investigate gene expression in Tribolium castaneum exposed to Beauveria bassiana, reference genes for qPCR were evaluated. Of these, beta-actin, alpha-tubulin, and RPS6 were not stable. The most stable were ribosomal protein genes, RPS3, RPS18, and RPL13a. Syntaxin1, syntaxin6, and E-cadherin may be appropriate for some experimental systems. Published by Elsevier B.V.

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Year:  2009        PMID: 20026205     DOI: 10.1016/j.mimet.2009.12.007

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  60 in total

1.  Patterning of the adult mandibulate mouthparts in the red flour beetle, Tribolium castaneum.

Authors:  David R Angelini; Frank W Smith; Ariel C Aspiras; Moto Kikuchi; Elizabeth L Jockusch
Journal:  Genetics       Date:  2011-11-30       Impact factor: 4.562

2.  Fine-scale analysis of parasite resistance genes in the red flour beetle, Tribolium castaneum.

Authors:  Daibin Zhong; Aditi Pai; Mei-Hui Wang; Naomi Keech; Guiyun Yan
Journal:  Genetics       Date:  2013-06-14       Impact factor: 4.562

3.  Alternatively spliced orcokinin isoforms and their functions in Tribolium castaneum.

Authors:  Hongbo Jiang; Hong Geun Kim; Yoonseong Park
Journal:  Insect Biochem Mol Biol       Date:  2015-07-30       Impact factor: 4.714

4.  Sodium solute symporter and cadherin proteins act as Bacillus thuringiensis Cry3Ba toxin functional receptors in Tribolium castaneum.

Authors:  Estefanía Contreras; Michael Schoppmeier; M Dolores Real; Carolina Rausell
Journal:  J Biol Chem       Date:  2013-05-03       Impact factor: 5.157

5.  Validation of reference genes for quantitative expression analysis by real-time rt-PCR in four lepidopteran insects.

Authors:  Xiaolu Teng; Zan Zhang; Guiling He; Liwen Yang; Fei Li
Journal:  J Insect Sci       Date:  2012       Impact factor: 1.857

6.  RNAi phenotypes are influenced by the genetic background of the injected strain.

Authors:  Peter Kitzmann; Jonas Schwirz; Christian Schmitt-Engel; Gregor Bucher
Journal:  BMC Genomics       Date:  2013-01-16       Impact factor: 3.969

7.  Rapid male-specific regulatory divergence and down regulation of spermatogenesis genes in Drosophila species hybrids.

Authors:  Jennifer Ferguson; Suzanne Gomes; Alberto Civetta
Journal:  PLoS One       Date:  2013-04-11       Impact factor: 3.240

8.  Validation of reference genes in Solenopsis invicta in different developmental stages, castes and tissues.

Authors:  Daifeng Cheng; Zhiling Zhang; Xiaofang He; Guangwen Liang
Journal:  PLoS One       Date:  2013-02-28       Impact factor: 3.240

9.  Proteome response of Tribolium castaneum larvae to Bacillus thuringiensis toxin producing strains.

Authors:  Estefanía Contreras; Carolina Rausell; M Dolores Real
Journal:  PLoS One       Date:  2013-01-25       Impact factor: 3.240

10.  Validation of reference genes for expression analysis by quantitative real-time PCR in Leptinotarsa decemlineata (Say).

Authors:  Xiao-Qin Shi; Wen-Chao Guo; Pin-Jun Wan; Li-Tao Zhou; Xiang-Liang Ren; Tursun Ahmat; Kai-Yun Fu; Guo-Qing Li
Journal:  BMC Res Notes       Date:  2013-03-13
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