Chemical composition analysis of soybean oligosaccharides and its effect on ATPase activities in hyperlipidemic rats.

A water-soluble oligosaccharide was isolated from the water extract of soybean, which is widely grown worldwide due to its nutritional benefits of a full range of amino acids and a high protein content. In this study, we analysed chemical composition of soybean oligosaccharides (SBOS) using high performance liquid chromatography (HPLC). The results obtained were compared with the following sugar standards: mannose, galactose, xylose, arabinose, fucose and glucose. Results showed that SBOS was composed of galactose (65.3%), mannose (15.6%), fructose (7.8%) and glucose (8.7%). The pharmacological action of SBOS has been studied in hyperlipidemic rats fed high-fat diet. In hyperlipidemic rats, feeding with SBOS (150 and 300 mg/kg b.w.) exerted a pharmacological effect as assessed by reversal of plasma levels of lactic acid, nitric oxide (NO), vascular endothelial growth factor (VEGF) levels, and heart Na+-K+-ATPase, Ca2+-ATPase and Mg2+-ATPase activities. These results indicated that SBOS may have beneficial effects on the therapy of some heart diseases, such as coronary artery diseases. 2009 Elsevier B.V. All rights reserved.