BACKGROUND: Intraplaque haemorrhage has been shown to be important in necrotic core enlargement. Immunolocalisation of fibrin within progressive stages of plaque progression has not been extensively studied. METHODS: Histological sections (n = 74) of human coronary arteries were stained immunohistochemically for fibrin II, red blood cell antigen (glycophorin A), and CD31. Plaques were chosen to represent a range of lesions [6 adaptive intimal thickening, AIT (AHA grade I); 4 intimal xanthomas (AHA grade II), 19 pathologic intimal thickening, PIT (AHA grade III, or pre-atheroma); 34 fibroatheromas, FA (AHA grade IV and V); and 11 thin cap fibroatheromas (TCFA, AHA grade IV)]. RESULTS: Fibrin was generally absent in the intima of AIT and PIT, with moderate staining in cores of early FA (2.6 +/- 0.3). All late FA and TCFA demonstrated intracore fibrin, with mean scores of 2.9 +/- 0.3 and 3.0 +/- 0.3, respectively. Intimal vasa vasorum counts increased with intimal fibrin score (p < 0.0001); in 68% of cores with fibrin staining, there was minimal or no evidence of red cell breakdown. CONCLUSIONS: Fibrin in necrotic cores is present proportional to intraplaque vasa vasorum and before red cells, suggesting leakage of vessels before frank intraplaque haemorrhage. Fibrin may play a role in the bridge between pre-atheroma and atheroma.
BACKGROUND: Intraplaque haemorrhage has been shown to be important in necrotic core enlargement. Immunolocalisation of fibrin within progressive stages of plaque progression has not been extensively studied. METHODS: Histological sections (n = 74) of human coronary arteries were stained immunohistochemically for fibrin II, red blood cell antigen (glycophorin A), and CD31. Plaques were chosen to represent a range of lesions [6 adaptive intimal thickening, AIT (AHA grade I); 4 intimal xanthomas (AHA grade II), 19 pathologic intimal thickening, PIT (AHA grade III, or pre-atheroma); 34 fibroatheromas, FA (AHA grade IV and V); and 11 thin cap fibroatheromas (TCFA, AHA grade IV)]. RESULTS: Fibrin was generally absent in the intima of AIT and PIT, with moderate staining in cores of early FA (2.6 +/- 0.3). All late FA and TCFA demonstrated intracore fibrin, with mean scores of 2.9 +/- 0.3 and 3.0 +/- 0.3, respectively. Intimal vasa vasorum counts increased with intimal fibrin score (p < 0.0001); in 68% of cores with fibrin staining, there was minimal or no evidence of red cell breakdown. CONCLUSIONS: Fibrin in necrotic cores is present proportional to intraplaque vasa vasorum and before red cells, suggesting leakage of vessels before frank intraplaque haemorrhage. Fibrin may play a role in the bridge between pre-atheroma and atheroma.
Authors: Romana Meletta; Nicole Borel; Paul Stolzmann; Alberto Astolfo; Jan Klohs; Marco Stampanoni; Markus Rudin; Roger Schibli; Stefanie D Krämer; Adrienne Müller Herde Journal: Int J Cardiovasc Imaging Date: 2015-07-16 Impact factor: 2.357
Authors: Xueming Wu; Niranjan Balu; Wen Li; Yong Chen; Xiaoyue Shi; China M Kummitha; Xin Yu; Chun Yuan; Zheng-Rong Lu Journal: Am J Nucl Med Mol Imaging Date: 2013-09-19
Authors: Dominik Nörenberg; Hans U Ebersberger; Gerd Diederichs; Bernd Hamm; René M Botnar; Marcus R Makowski Journal: Eur Radiol Date: 2015-07-03 Impact factor: 5.315
Authors: Alkystis Phinikaridou; Marcelo E Andia; Sara Lacerda; Silvia Lorrio; Marcus R Makowski; René M Botnar Journal: Molecules Date: 2013-11-13 Impact factor: 4.411