Literature DB >> 20023086

Optimization of RNA extraction for PCR quantification of aromatic compound degradation genes.

Weidong Kong1, Cindy H Nakatsu.   

Abstract

Seven different bacterial strains and primer sets and a mixed community were used to evaluate the use of reverse transcriptase quantitative PCR (Q-PCR) and Q-PCR of oxygenase genes to assess various approaches for monitoring the bioremediation of polluted sites. Differences in maximum activity were seen when different RNA extraction kits were compared.

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Year:  2009        PMID： 20023086      PMCID： PMC2820950          DOI： 10.1128/AEM.01939-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  13 in total

1.  Development of catechol 2,3-dioxygenase-specific primers for monitoring bioremediation by competitive quantitative PCR.

Authors:  M B Mesarch; C H Nakatsu; L Nies
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

2.  Analyses of microbial activity in biomass-recycle reactors using denaturing gradient gel electrophoresis of 16S rDNA and 16S rRNA PCR products.

Authors:  Christine A Morgan; Andre Hudson; Allan Konopka; Cindy H Nakatsu
Journal:  Can J Microbiol       Date:  2002-04       Impact factor: 2.419

3.  Detection and enumeration of aromatic oxygenase genes by multiplex and real-time PCR.

Authors:  Brett R Baldwin; Cindy H Nakatsu; Loring Nies
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

4.  Quantification of dissimilatory (bi)sulphite reductase gene expression in Desulfobacterium autotrophicum using real-time RT-PCR.

Authors:  Lev N Neretin; Axel Schippers; Annelie Pernthaler; Knut Hamann; Rudolf Amann; Bo Barker Jørgensen
Journal:  Environ Microbiol       Date:  2003-08       Impact factor: 5.491

5.  Real-time PCR quantification of rbcL (ribulose-1,5-bisphosphate carboxylase/oxygenase) mRNA in diatoms and pelagophytes.

Authors:  B Wawrik; J H Paul; F R Tabita
Journal:  Appl Environ Microbiol       Date:  2002-08       Impact factor: 4.792

6.  Temporal expression of respiratory genes in an enrichment culture containing Dehalococcoides ethenogenes.

Authors:  Brian G Rahm; Robert M Morris; Ruth E Richardson
Journal:  Appl Environ Microbiol       Date:  2006-08       Impact factor: 4.792

7.  Quantification of aromatic oxygenase genes to evaluate enhanced bioremediation by oxygen releasing materials at a gasoline-contaminated site.

Authors:  Jennifer Nebe; Brett R Baldwin; Raymond L Kassab; Loring Nies; Cindy H Nakatsu
Journal:  Environ Sci Technol       Date:  2009-03-15       Impact factor: 9.028

Review 8.  Growth kinetics of suspended microbial cells: from single-substrate-controlled growth to mixed-substrate kinetics.

Authors:  K Kovárová-Kovar; T Egli
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

9.  Simultaneous extraction from bacterioplankton of total RNA and DNA suitable for quantitative structure and function analyses.

Authors:  Markus G Weinbauer; Ingo Fritz; Dirk F Wenderoth; Manfred G Höfle
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

10.  Enumeration of aromatic oxygenase genes to evaluate monitored natural attenuation at gasoline-contaminated sites.

Authors:  Brett R Baldwin; Cindy H Nakatsu; Loring Nies
Journal:  Water Res       Date:  2007-08-06       Impact factor: 11.236
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  3 in total

1.  Diversity and expression of RubisCO genes in a perennially ice-covered Antarctic lake during the polar night transition.

Authors:  Weidong Kong; David C Ream; John C Priscu; Rachael M Morgan-Kiss
Journal:  Appl Environ Microbiol       Date:  2012-04-06       Impact factor: 4.792

2.  Benzene Degradation by a Variovorax Species within a Coal Tar-Contaminated Groundwater Microbial Community.

Authors:  Kevin M Posman; Christopher M DeRito; Eugene L Madsen
Journal:  Appl Environ Microbiol       Date:  2017-02-01       Impact factor: 4.792

3.  Mycoplasma iowae: relationships among oxygen, virulence, and protection from oxidative stress.

Authors:  Rachel E Pritchard; Mitchell F Balish
Journal:  Vet Res       Date:  2015-03-21       Impact factor: 3.683
  3 in total

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