Rapid and direct magnetization of GFP-reporter yeast for micro-screening systems.

Saccharomyces cerevisiae containing fluorescent markers are ideal candidates for applications in microfluidic screening systems as fluorescence signal is emitted without the need of additional reagents. Here we develop a method for magnetic functionalization of such cells which allows their handling and immobilization in micro-screening devices. After exposure of the magnetized GFP-reporter yeast (GreenScreen) to a genotoxic compound, the fluorescence emission was detected using fluorescent spectrometer and an Epi-fluorescent microscope. Results demonstrate that GFP production and fluorescence emission is not altered by their magnetic functionalization, indicating its potential employment on biosensors, bioreactors and micro-screening studies. (c) 2009 Elsevier B.V. All rights reserved.