Literature DB >> 20018359

Freezing dog semen in presence of the antioxidant butylated hydroxytoluene improves postthaw sperm membrane integrity.

V R Neagu1, B Macías García, C Salazar Sandoval, A Morillo Rodríguez, C Ortega Ferrusola, L González Fernández, J A Tapia, F J Peña.   

Abstract

In an attempt to evaluate the protective effect of a lipid-soluble antioxidant (butylated hydroxytoluene; BHT), semen from four dogs (Canis familiaris) was frozen in two different extenders (Uppsala or INRA-96 plus glycerol) with or without 1mM BHT. Sperm membrane integrity using flow cytometry and motility using a computerized system were evaluated in each experimental group. The Uppsala extender was superior in all aspects of sperm function. The percentage of sperm membranes was significantly higher in semen samples frozen in presence of BHT. Our results suggest that the Uppsala extender can be improved with the addition of BHT. Copyright 2010 Elsevier Inc. All rights reserved.

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Year:  2009        PMID： 20018359     DOI： 10.1016/j.theriogenology.2009.10.021

Source DB:  PubMed          Journal:  Theriogenology        ISSN： 0093-691X            Impact factor:   2.740


  8 in total

1.  Effects of rapid cooling prior to freezing on the quality of canine cryopreserved spermatozoa.

Authors:  Carmen Rodenas; Inmaculada Parrilla; Jordi Roca; Emilio Arsenio Martinez; Xiomara Lucas
Journal:  J Reprod Dev       Date:  2014-07-21       Impact factor: 2.214

2.  Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs.

Authors:  Hiroshi Suzuki; Hiroyuki Watanabe; Yasuyuki Abe
Journal:  J Reprod Dev       Date:  2021-11-29       Impact factor: 2.214

3.  Oligomeric Proanthocyanidins and Bamboo Leaf Flavonoids Improve the Quality of Bull Semen Cryopreservation.

Authors:  Hongtao Wang; Ping Lu; Zhiqiang Li; Chongshan Yuan; Hongyu Liu; Jing Zhao; Wenfa Lu; Jun Wang
Journal:  Molecules       Date:  2022-02-08       Impact factor: 4.411

4.  The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa.

Authors:  Maria Eberhardt; Sylwia Prochowska; Anna M Duszewska; Ann Van Soom; Wanda Olech; Wojciech Niżański
Journal:  BMC Vet Res       Date:  2022-08-10       Impact factor: 2.792

5.  Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility.

Authors:  María Plaza Davila; Patricia Martin Muñoz; Jose A Tapia; Cristina Ortega Ferrusola; Carolina Balao da Silva C; Fernando J Peña
Journal:  PLoS One       Date:  2015-09-25       Impact factor: 3.240

6.  Male Pronuclear Formation using Dog Sperm Derived from Ectopic Testicular Xenografts, Testis, and Epididymis.

Authors:  Abolfazl Shirazi; Asma Khadivi; Naser Shams-Esfandabadi
Journal:  Avicenna J Med Biotechnol       Date:  2014-07

7.  Effect of supplementation of butylated hydroxytoluene on post-thaw sperm viability, motility and membrane integrity of Hariana bulls.

Authors:  Akhil Patel; Atul Saxena; Dilip Kumar Swain; Dushyant Yadav; Sanjay Singh Yadav; Abhishek Kumar; Anuj Kumar
Journal:  Vet World       Date:  2015-06-30

Review 8.  Overview on the Antioxidants, Egg Yolk Alternatives, and Mesenchymal Stem Cells and Derivatives Used in Canine Sperm Cryopreservation.

Authors:  Feriel Yasmine Mahiddine; Min-Jung Kim
Journal:  Animals (Basel)       Date:  2021-06-28       Impact factor: 2.752
  8 in total

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