Literature DB >> 2001733

Cloning and sequencing of Lol pI, the major allergenic protein of rye-grass pollen.

I J Griffith1, P M Smith, J Pollock, P Theerakulpisut, A Avjioglu, S Davies, T Hough, M B Singh, R J Simpson, L D Ward.   

Abstract

We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.

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Year:  1991        PMID： 2001733     DOI： 10.1016/0014-5793(91)80151-r

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  18 in total

1.  Pollination modulates expression of the PPAL gene, a pistil-specific beta-expansin.

Authors:  Mario Pezzotti; Richard Feron; Celestina Mariani
Journal:  Plant Mol Biol       Date:  2002-05       Impact factor: 4.076

2.  Isolation and developmental expression of Bcp1, an anther-specific cDNA clone in Brassica campestris.

Authors:  P Theerakulpisut; H Xu; M B Singh; J M Pettitt; R B Knox
Journal:  Plant Cell       Date:  1991-10       Impact factor: 11.277

3.  Class B beta-expansins are needed for pollen separation and stigma penetration.

Authors:  Elene R Valdivia; Andrew G Stephenson; Daniel M Durachko; Daniel Cosgrove
Journal:  Sex Plant Reprod       Date:  2009-06-04

Review 4.  Modulation of allergen-specific antibody responses by T-cell-based peptide vaccine(s). Principles and potential.

Authors:  S S Mohapatra
Journal:  Clin Rev Allergy       Date:  1994

5.  Molecular basis of cross-reactivity among allergen-specific human T cells: T-cell receptor V alpha gene usage and epitope structure.

Authors:  S S Mohapatra; S Mohapatra; M Yang; A A Ansari; P Parronchi; E Maggi; S Romagnani
Journal:  Immunology       Date:  1994-01       Impact factor: 7.397

6.  Group I allergens of grass pollen as cell wall-loosening agents.

Authors:  D J Cosgrove; P Bedinger; D M Durachko
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-10       Impact factor: 11.205

7.  A novel glycine-rich/hydrophobic 16 kDa polypeptide gene from tobacco: similarity to proline-rich protein genes and its wound-inducible and developmentally regulated expression.

Authors:  E Yasuda; H Ebinuma; H Wabiko
Journal:  Plant Mol Biol       Date:  1997-03       Impact factor: 4.076

8.  Antisense-mediated silencing of a gene encoding a major ryegrass pollen allergen.

Authors:  P L Bhalla; I Swoboda; M B Singh
Journal:  Proc Natl Acad Sci U S A       Date:  1999-09-28       Impact factor: 11.205

9.  A cDNA from pea petals with sequence similarity to pollen allergen, cytokinin-induced and genetic tumour-specific genes: identification of a new family of related sequences.

Authors:  A J Michael
Journal:  Plant Mol Biol       Date:  1996-01       Impact factor: 4.076

10.  T cell epitopes of the major fraction of rye grass Lolium perenne (Lol p I) defined using overlapping peptides in vitro and in vivo. I. Isoallergen clone1A.

Authors:  G A Bungy Poor Fard; Y Latchman; S Rodda; M Geysen; I Roitt; J Brostoff
Journal:  Clin Exp Immunol       Date:  1993-10       Impact factor: 4.330
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