Literature DB >> 20013202

Purification and characterization of biologically active peptides from spider venoms.

Alexander A Vassilevski1, Sergey A Kozlov, Tsezi A Egorov, Eugene V Grishin.   

Abstract

Spider venoms represent invaluable sources of biologically active compounds suitable for use in life science research and also having a significant potential for biotechnology and therapeutic applications. The methods reported herewith are based on our long experience of spider venom fractionation and peptides purification. We routinely screen new peptides for antimicrobial and insecticidal activities and our detailed protocols are also reported here. So far these have been tested on species of Central Asian and European spiders from the families Agelenidae, Eresidae, Gnaphosidae, Lycosidae, Miturgidae, Oxyopidae, Philodromidae, Pisauridae, Segestriidae, Theridiidae, Thomisidae, and Zodariidae. The reported protocols should be easily adaptable for use with other arthropod species.

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Year:  2010        PMID: 20013202     DOI: 10.1007/978-1-60761-535-4_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  10 in total

1.  Novel class of spider toxin: active principle from the yellow sac spider Cheiracanthium punctorium venom is a unique two-domain polypeptide.

Authors:  Alexander A Vassilevski; Irina M Fedorova; Ekaterina E Maleeva; Yuliya V Korolkova; Svetlana S Efimova; Olga V Samsonova; Ludmila V Schagina; Alexei V Feofanov; Lev G Magazanik; Eugene V Grishin
Journal:  J Biol Chem       Date:  2010-07-24       Impact factor: 5.157

2.  Unique bell-shaped voltage-dependent modulation of Na+ channel gating by novel insect-selective toxins from the spider Agelena orientalis.

Authors:  Bert Billen; Alexander Vassilevski; Anton Nikolsky; Sarah Debaveye; Jan Tytgat; Eugene Grishin
Journal:  J Biol Chem       Date:  2010-04-12       Impact factor: 5.157

3.  Structural similarity between defense peptide from wheat and scorpion neurotoxin permits rational functional design.

Authors:  Antonina A Berkut; Dinara R Usmanova; Steve Peigneur; Peter B Oparin; Konstantin S Mineev; Tatyana I Odintsova; Jan Tytgat; Alexander S Arseniev; Eugene V Grishin; Alexander A Vassilevski
Journal:  J Biol Chem       Date:  2014-03-26       Impact factor: 5.157

4.  Variability of Potassium Channel Blockers in Mesobuthus eupeus Scorpion Venom with Focus on Kv1.1: AN INTEGRATED TRANSCRIPTOMIC AND PROTEOMIC STUDY.

Authors:  Alexey I Kuzmenkov; Alexander A Vassilevski; Kseniya S Kudryashova; Oksana V Nekrasova; Steve Peigneur; Jan Tytgat; Alexey V Feofanov; Mikhail P Kirpichnikov; Eugene V Grishin
Journal:  J Biol Chem       Date:  2015-03-19       Impact factor: 5.157

Review 5.  Leveraging orthogonal mass spectrometry based strategies for comprehensive sequencing and characterization of ribosomal antimicrobial peptide natural products.

Authors:  Tessa B Moyer; Nicole C Parsley; Patric W Sadecki; Wyatt J Schug; Leslie M Hicks
Journal:  Nat Prod Rep       Date:  2020-09-15       Impact factor: 13.423

6.  Expression of VEGF and Flk-1 and Flt-1 receptors during blood-brain barrier (BBB) impairment following Phoneutria nigriventer spider venom exposure.

Authors:  Monique C P Mendonça; Edilene S Soares; Leila M Stávale; Catarina Rapôso; Andressa Coope; Evanguedes Kalapothakis; Maria Alice da Cruz-Höfling
Journal:  Toxins (Basel)       Date:  2013-12-18       Impact factor: 4.546

7.  Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity.

Authors:  Efe Erdeş; Tuğba Somay Doğan; Ilhan Coşar; Tarık Danışman; Kadir Boğaç Kunt; Tamay Seker; Meral Yücel; Can Ozen
Journal:  J Venom Anim Toxins Incl Trop Dis       Date:  2014-11-03

8.  Apamin structure and pharmacology revisited.

Authors:  Alexey I Kuzmenkov; Steve Peigneur; Joshua A Nasburg; Konstantin S Mineev; Maxim V Nikolaev; Ernesto Lopes Pinheiro-Junior; Alexander S Arseniev; Heike Wulff; Jan Tytgat; Alexander A Vassilevski
Journal:  Front Pharmacol       Date:  2022-09-16       Impact factor: 5.988

9.  Biological and Enzymatic Characterization of Proteases from Crude Venom of the Ant Odontomachus bauri.

Authors:  Mariana Ferreira Silva; Caroline Martins Mota; Vanessa dos Santos Miranda; Amanda de Oliveira Cunha; Maraísa Cristina Silva; Karinne Spirandelli Carvalho Naves; Fábio de Oliveira; Deise Aparecida de Oliveira Silva; Tiago Wilson Patriarca Mineo; Fernanda Maria Santiago
Journal:  Toxins (Basel)       Date:  2015-11-30       Impact factor: 4.546

10.  ω-Tbo-IT1-New Inhibitor of Insect Calcium Channels Isolated from Spider Venom.

Authors:  Alexander N Mikov; Irina M Fedorova; Natalia N Potapieva; Ekaterina E Maleeva; Yaroslav A Andreev; Alexey V Zaitsev; Kira K Kim; Eduard V Bocharov; Timur N Bozin; Dmitry A Altukhov; Alexey V Lipkin; Sergey A Kozlov; Denis B Tikhonov; Eugene V Grishin
Journal:  Sci Rep       Date:  2015-11-27       Impact factor: 4.379

  10 in total

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