Literature DB >> 20012843

Tissue disaggregation.

Robert E Cunningham1.   

Abstract

A conventional flow cytometry procedure for creating single cell suspensions is provided. Methods of tissue disaggregation to create single-cell suspensions from formalin fixed tissue are also given. Chemical, mechanical, and enzymatic multistep methods for separating single cells from tissue are discussed. These procedures are very tissue dependent.

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Year:  2010        PMID： 20012843     DOI： 10.1007/978-1-59745-324-0_32

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Automated-Mechanical Procedure Compared to Gentle Enzymatic Tissue Dissociation in Cell Function Studies.

Authors:  Mariele Montanari; Sabrina Burattini; Caterina Ciacci; Patrizia Ambrogini; Silvia Carloni; Walter Balduini; Daniele Lopez; Giovanna Panza; Stefano Papa; Barbara Canonico
Journal:  Biomolecules       Date:  2022-05-14

Review 2.  Technologies for deriving primary tumor cells for use in personalized cancer therapy.

Authors:  Abhisek Mitra; Lopa Mishra; Shulin Li
Journal:  Trends Biotechnol       Date:  2013-04-16       Impact factor: 19.536

3.  An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells.

Authors:  Jon G Quatromoni; Sunil Singhal; Pratik Bhojnagarwala; Wayne W Hancock; Steven M Albelda; Evgeniy Eruslanov
Journal:  J Leukoc Biol       Date:  2014-10-30       Impact factor: 4.962

Review 4.  Management and potentialities of primary cancer cultures in preclinical and translational studies.

Authors:  Giacomo Miserocchi; Laura Mercatali; Chiara Liverani; Alessandro De Vita; Chiara Spadazzi; Federica Pieri; Alberto Bongiovanni; Federica Recine; Dino Amadori; Toni Ibrahim
Journal:  J Transl Med       Date:  2017-11-07       Impact factor: 5.531
  4 in total

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