Literature DB >> 2001256

Gly429 is the major determinant of uncompetitive inhibition of human germ cell alkaline phosphatase by L-leucine.

C Hummer1, J L Millán.   

Abstract

The catalytic activity of human placental alkaline phosphatase (PLAP) and germ cell alkaline phosphatase (GCAP) can be inhibited, through an uncompetitive mechanism, by L-Phe. GCAP is also selectively inhibited by L-Leu. Site-directed mutagenesis of five of the 12 residues which are different in PLAP and GCAP revealed that Gly429 is the primary determinant of GCAP inhibition by L-Leu, and Ser84 and Leu297 play a modulatory role in the inhibition.

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Year:  1991        PMID： 2001256      PMCID： PMC1149924          DOI： 10.1042/bj2740091

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  28 in total

1.  L-phenylalanine: an organ specific, stereospecific inhibitor of human intestinal alkaline phosphatase.

Authors:  W H FISHMAN; S GREEN; N I INGLIS
Journal:  Nature       Date:  1963-05-18       Impact factor: 49.962

2.  L-leucine, a specific inhibitor of a rare human placental alkaline phosphatase phenotype.

Authors:  G J Doellgast; W H Fishman
Journal:  Nature       Date:  1976 Jan 1-8       Impact factor: 49.962

Review 3.  Perspectives on alkaline phosphatase isoenzymes.

Authors:  W H Fishman
Journal:  Am J Med       Date:  1974-05       Impact factor: 4.965

4.  Studies on alkaline phosphatase. Inhibition of human-placental phosphoryl phosphatase by L-phenylalanine.

Authors:  D A Byers; H N Fernley; P G Walker
Journal:  Eur J Biochem       Date:  1972-09-18

5.  L-Homoarginine. An organ-specific, uncompetitive inhibitor of human liver and bone alkaline phosphohydrolases.

Authors:  C W Lin; W H Fishman
Journal:  J Biol Chem       Date:  1972-05-25       Impact factor: 5.157

6.  Organ-specific inhibition of human alkaline phosphatase isoenzymes of liver, bone, intestine and placenta; L-phenylalanine, L-tryptophan and L homoarginine.

Authors:  W H Fishman; H G Sie
Journal:  Enzymologia       Date:  1971-09-30

7.  Differential inhibition of the products of the human alkaline phosphatase loci.

Authors:  R A Mulivor; L I Plotkin; H Harris
Journal:  Ann Hum Genet       Date:  1978-07       Impact factor: 1.670

8.  On the mechanism of inhibition of intestinal alkaline phosphatase by L-phenylalanine. I. Kinetic studies.

Authors:  N K Ghosh; W H Fishman
Journal:  J Biol Chem       Date:  1966-06-10       Impact factor: 5.157

9.  L-p-Bromotetramisole, a new reagent for use in measuring placental or intestinal isoenzymes of alkaline phosphatase in human serum.

Authors:  H Van Belle; M E De Broe; R J Wieme
Journal:  Clin Chem       Date:  1977-03       Impact factor: 8.327

10.  Hypophosphatasia (adult form): quantitation of serum alkaline phosphatase isoenzyme activity in a large kindred.

Authors:  J L Millán; M P Whyte; L V Avioli; W H Fishman
Journal:  Clin Chem       Date:  1980-06       Impact factor: 8.327
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  8 in total

1.  Cloning and expression of the bovine intestinal alkaline phosphatase gene: biochemical characterization of the recombinant enzyme.

Authors:  H Weissig; A Schildge; M F Hoylaerts; M Iqbal; J L Millán
Journal:  Biochem J       Date:  1993-03-01       Impact factor: 3.857

2.  Molecular mechanism of uncompetitive inhibition of human placental and germ-cell alkaline phosphatase.

Authors:  M F Hoylaerts; T Manes; J L Millán
Journal:  Biochem J       Date:  1992-08-15       Impact factor: 3.857

3.  Phosphate binding in the active site of alkaline phosphatase and the interactions of 2-nitrosoacetophenone with alkaline phosphatase-induced small structural changes.

Authors:  Le Zhang; René Buchet; Gérard Azzar
Journal:  Biophys J       Date:  2004-06       Impact factor: 4.033

4.  Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor.

Authors:  Deepti Saini; Mrinalini Kala; Vishal Jain; Subrata Sinha
Journal:  BMC Biotechnol       Date:  2005-12-22       Impact factor: 2.563

5.  Alkaline Phosphatases : Structure, substrate specificity and functional relatedness to other members of a large superfamily of enzymes.

Authors:  José Luis Millán
Journal:  Purinergic Signal       Date:  2006-06-17       Impact factor: 3.765

Review 6.  Hypophosphatasia: A Unique Disorder of Bone Mineralization.

Authors:  Juan Miguel Villa-Suárez; Cristina García-Fontana; Francisco Andújar-Vera; Sheila González-Salvatierra; Tomás de Haro-Muñoz; Victoria Contreras-Bolívar; Beatriz García-Fontana; Manuel Muñoz-Torres
Journal:  Int J Mol Sci       Date:  2021-04-21       Impact factor: 5.923

Review 7.  Alkaline Phosphatase and Hypophosphatasia.

Authors:  José Luis Millán; Michael P Whyte
Journal:  Calcif Tissue Int       Date:  2015-11-21       Impact factor: 4.333

Review 8.  TNAP as a therapeutic target for cardiovascular calcification: a discussion of its pleiotropic functions in the body.

Authors:  Claudia Goettsch; Agnieszka Strzelecka-Kiliszek; Laurence Bessueille; Thibaut Quillard; Laura Mechtouff; Slawomir Pikula; Emmanuelle Canet-Soulas; Jose Luis Millan; Caroline Fonta; David Magne
Journal:  Cardiovasc Res       Date:  2022-01-07       Impact factor: 10.787
  8 in total

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