Literature DB >> 2001247

Stability of mutant actins.

D R Drummond1, E S Hennessey, J C Sparrow.   

Abstract

Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in stability, and E316K was the least stable. The causes of the differences are discussed and contrasted with the behaviour of the mutants in vivo, where E316K has normal stability and V339I is the least stable.

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Year:  1991        PMID： 2001247      PMCID： PMC1149953          DOI： 10.1042/bj2740301

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

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Authors:  T E Creighton
Journal:  J Mol Biol       Date:  1979-04-05       Impact factor: 5.469

2.  Alteration in crossbridge kinetics caused by mutations in actin.

Authors:  D R Drummond; M Peckham; J C Sparrow; D C White
Journal:  Nature       Date:  1990-11-29       Impact factor: 49.962

3.  Kinetic study of protein unfolding and refolding using urea gradient electrophoresis.

Authors:  T E Creighton
Journal:  J Mol Biol       Date:  1980-02-15       Impact factor: 5.469

Review 4.  Intracellular protein degradation in mammalian and bacterial cells.

Authors:  A L Goldberg; J F Dice
Journal:  Annu Rev Biochem       Date:  1974       Impact factor: 23.643

Review 5.  Intracellular protein degradation in mammalian and bacterial cells: Part 2.

Authors:  A L Goldberg; A C St John
Journal:  Annu Rev Biochem       Date:  1976       Impact factor: 23.643

Review 6.  Gel electrophoresis in studies of protein conformation and folding.

Authors:  D P Goldenberg; T E Creighton
Journal:  Anal Biochem       Date:  1984-04       Impact factor: 3.365

7.  Turnover of excess hemoglobin alpha chains in beta-thalassemic cells is ATP-dependent.

Authors:  J R Shaeffer
Journal:  J Biol Chem       Date:  1983-11-10       Impact factor: 5.157

8.  Is protein turnover thermodynamically controlled?

Authors:  G McLendon; E Radany
Journal:  J Biol Chem       Date:  1978-09-25       Impact factor: 5.157

9.  Purification of muscle actin.

Authors:  J D Pardee; J A Spudich
Journal:  Methods Enzymol       Date:  1982       Impact factor: 1.600

10.  Synthesis and turnover of cytoskeletal proteins in cultured astrocytes.

Authors:  F C Chiu; J E Goldman
Journal:  J Neurochem       Date:  1984-01       Impact factor: 5.372
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  4 in total

1.  A mutant of Arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast.

Authors:  J L Morrell; M Morphew; K L Gould
Journal:  Mol Biol Cell       Date:  1999-12       Impact factor: 4.138

Review 2.  Molecular genetics of actin function.

Authors:  E S Hennessey; D R Drummond; J C Sparrow
Journal:  Biochem J       Date:  1993-05-01       Impact factor: 3.857

3.  Actomyosin kinetics and in vitro motility of wild-type Drosophila actin and the effects of two mutations in the Act88F gene.

Authors:  M Anson; D R Drummond; M A Geeves; E S Hennessey; M D Ritchie; J C Sparrow
Journal:  Biophys J       Date:  1995-05       Impact factor: 4.033

4.  ADP-ribosylation of Drosophila indirect-flight-muscle actin and arthrin by Clostridium botulinum C2 toxin and Clostridium perfringens iota toxin.

Authors:  I Just; E S Hennessey; D R Drummond; K Aktories; J C Sparrow
Journal:  Biochem J       Date:  1993-04-15       Impact factor: 3.857
  4 in total

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