Literature DB >> 2001230

The effects of systematic errors on the analysis of irreversible enzyme inhibition progress curves.

P Gray1.   

Abstract

The effects of errors in Km and Vmax. on the determination of the kinetic constants for the irreversible inhibition of acetylcholinesterase were determined. The results show that small errors in the values of Km and Vmax. give rise to large errors in the fitted parameters k2 and Kd. The problem may be minimized for multiple progress curves by selecting an appropriate number and distribution of inhibitor concentrations.

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Year:  1991        PMID： 2001230      PMCID： PMC1149936          DOI： 10.1042/bj2740181

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  Analysis of kinetic data for irreversible enzyme inhibition.

Authors:  P J Gray; R G Duggleby
Journal:  Biochem J       Date:  1989-01-15       Impact factor: 3.857

2.  Recording spectrophotometric method for determination of dissociation and phosphorylation constants for the inhibition of acetylcholinesterase by organophosphates in the presence of substrate.

Authors:  G J Hart; R D O'Brien
Journal:  Biochemistry       Date:  1973-07-17       Impact factor: 3.162

3.  Acceleration by free carbamate of the spontaneous reactivation of carbamylated acetylcholinesterase.

Authors:  W K Berry
Journal:  Biochem Pharmacol       Date:  1971-11       Impact factor: 5.858

4.  Determination of rate constants for the irreversible inhibition of acetylcholine esterase by continuously monitoring the substrate reaction in the presence of the inhibitor.

Authors:  W Liu; C L Tsou
Journal:  Biochim Biophys Acta       Date:  1986-03-28

5.  The interaction of carbamates with acetylcholinesterase.

Authors:  P Watts; R G Wilkinson
Journal:  Biochem Pharmacol       Date:  1977-04-15       Impact factor: 5.858

6.  Kinetics for the inhibition of acetylcholinesterase from the electric eel by some organophosphates and carbamates.

Authors:  A Forsberg; G Puu
Journal:  Eur J Biochem       Date:  1984-04-02

7.  Carbamylated acetylcholinesterase: acceleration of decarbamylation by bispyridinium oximes.

Authors:  R M Dawson; M Poretski
Journal:  Biochem Pharmacol       Date:  1985-12-15       Impact factor: 5.858

8.  Spontaneously reactivation of acetylcholinesterase inhibited by diisopropylfluorophosphate.

Authors:  K W Lanks; M J Seleznick
Journal:  Biochim Biophys Acta       Date:  1981-07-24

9.  Kinetic analysis of species difference in acetylcholinesterase sensitivity to organophosphate insecticides.

Authors:  C Wang; S D Murphy
Journal:  Toxicol Appl Pharmacol       Date:  1982-12       Impact factor: 4.219

10.  Determination of the rate constant of enzyme modification by measuring the substrate reaction in the presence of the modifier.

Authors:  W X Tian; C L Tsou
Journal:  Biochemistry       Date:  1982-03-02       Impact factor: 3.162
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