| Literature DB >> 20010549 |
Jens Benninghoff1, Angela Gritti, Matteo Rizzi, Giuseppe Lamorte, Robert J Schloesser, Angelika Schmitt, Stefanie Robel, Just Genius, Rainald Moessner, Peter Riederer, Husseini K Manji, Heinz Grunze, Dan Rujescu, Hans-Juergen Moeller, Klaus-Peter Lesch, Angelo Luigi Vescovi.
Abstract
Serotonin (5-HT) and the serotonergic system have recently been indicated as modulators of adult hippocampal neurogenesis. In this study, we evaluated the role of 5-HT on the functional features in neurospheres derived from adult neural stem cells (ANSC). We cultured neurospheres derived from mouse hippocampus in serum-free medium containing epidermal (EGF) and type-2 fibroblast growth factor (FGF2). Under these conditions ANSC expressed both isoforms of tryptophane-hydroxylase (TPH) and produced 5-HT. Blocking TPH function by para-chlorophenylalanine (PCPA) reduced ANSC proliferation, which was rescued by exogenous 5-HT. 5-HT action on ANSC was mediated predominantly by the serotonin receptor subtype 5-HT1A and, to a lesser extent, through the 5-HT2C (receptor) subtype, as shown by selectively antagonizing these receptors. Finally, we documented a 5-HT-induced increase of ANSC migration activity. In summary, we demonstrated a powerful serotonergic impact on ANSC functional features, which was mainly mediated by 5-HT1A receptors.Entities:
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Year: 2009 PMID： 20010549 PMCID： PMC3055363 DOI： 10.1038/npp.2009.181
Source DB: PubMed Journal: Neuropsychopharmacology ISSN： 0893-133X Impact factor: 7.853