Literature DB >> 20009564

Alcohol exposure alters DNA methylation profiles in mouse embryos at early neurulation.

Yunlong Liu1, Yokesh Balaraman, Guohua Wang, Kenneth P Nephew, Feng C Zhou.   

Abstract

Alcohol exposure during development can cause variable neurofacial deficit and growth retardation known as fetal alcohol spectrum disorders (FASD). The mechanism underlying FASD is not fully understood. However, alcohol, which is known to affect methyl donor metabolism, may induce aberrant epigenetic changes contributing to FASD. Using a tightly controlled whole-embryo culture, we investigated the effect of alcohol exposure (88mM) at early embryonic neurulation on genome-wide DNA methylation and gene expression in the C57BL/6 mouse. The DNA methylation landscape around promoter CpG islands at early mouse development was analyzed using MeDIP (methylated DNA immunoprecipitation) coupled with microarray (MeDIP-chip). At early neurulation, genes associated with high CpG promoters (HCP) had a lower ratio of methylation but a greater ratio of expression. Alcohol-induced alterations in DNA methylation were observed, particularly in genes on chromosomes 7, 10, and X; remarkably, a >10 fold increase in the number of genes with increased methylation on chromosomes 10 and X was observed in alcohol-exposed embryos with a neural tube defect phenotype compared to embryos without a neural tube defect. Significant changes in methylation were seen in imprinted genes, genes known to play roles in cell cycle, growth, apoptosis, cancer, and in a large number of genes associated with olfaction. Altered methylation was associated with significant (p<0.01) changes in expression for 84 genes. Sequenom EpiTYPER DNA methylation analysis was used for validation of the MeDIP-chip data. Increased methylation of genes known to play a role in metabolism (Cyp4f13) and decreased methylation of genes associated with development (Nlgn3, Elavl2, Sox21 and Sim1), imprinting (Igf2r) and chromatin (Hist1h3d) was confirmed. In a mouse model for FASD, we show for the first time that alcohol exposure during early neurulation can induce aberrant changes in DNA methylation patterns with associated changes in gene expression, which together may contribute to the observed abnormal fetal development.

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Year:  2009        PMID: 20009564      PMCID: PMC2805036          DOI: 10.4161/epi.4.7.9925

Source DB:  PubMed          Journal:  Epigenetics        ISSN: 1559-2294            Impact factor:   4.528


  66 in total

1.  Initial sequencing and comparative analysis of the mouse genome.

Authors:  Robert H Waterston; Kerstin Lindblad-Toh; Ewan Birney; Jane Rogers; Josep F Abril; Pankaj Agarwal; Richa Agarwala; Rachel Ainscough; Marina Alexandersson; Peter An; Stylianos E Antonarakis; John Attwood; Robert Baertsch; Jonathon Bailey; Karen Barlow; Stephan Beck; Eric Berry; Bruce Birren; Toby Bloom; Peer Bork; Marc Botcherby; Nicolas Bray; Michael R Brent; Daniel G Brown; Stephen D Brown; Carol Bult; John Burton; Jonathan Butler; Robert D Campbell; Piero Carninci; Simon Cawley; Francesca Chiaromonte; Asif T Chinwalla; Deanna M Church; Michele Clamp; Christopher Clee; Francis S Collins; Lisa L Cook; Richard R Copley; Alan Coulson; Olivier Couronne; James Cuff; Val Curwen; Tim Cutts; Mark Daly; Robert David; Joy Davies; Kimberly D Delehaunty; Justin Deri; Emmanouil T Dermitzakis; Colin Dewey; Nicholas J Dickens; Mark Diekhans; Sheila Dodge; Inna Dubchak; Diane M Dunn; Sean R Eddy; Laura Elnitski; Richard D Emes; Pallavi Eswara; Eduardo Eyras; Adam Felsenfeld; Ginger A Fewell; Paul Flicek; Karen Foley; Wayne N Frankel; Lucinda A Fulton; Robert S Fulton; Terrence S Furey; Diane Gage; Richard A Gibbs; Gustavo Glusman; Sante Gnerre; Nick Goldman; Leo Goodstadt; Darren Grafham; Tina A Graves; Eric D Green; Simon Gregory; Roderic Guigó; Mark Guyer; Ross C Hardison; David Haussler; Yoshihide Hayashizaki; LaDeana W Hillier; Angela Hinrichs; Wratko Hlavina; Timothy Holzer; Fan Hsu; Axin Hua; Tim Hubbard; Adrienne Hunt; Ian Jackson; David B Jaffe; L Steven Johnson; Matthew Jones; Thomas A Jones; Ann Joy; Michael Kamal; Elinor K Karlsson; Donna Karolchik; Arkadiusz Kasprzyk; Jun Kawai; Evan Keibler; Cristyn Kells; W James Kent; Andrew Kirby; Diana L Kolbe; Ian Korf; Raju S Kucherlapati; Edward J Kulbokas; David Kulp; Tom Landers; J P Leger; Steven Leonard; Ivica Letunic; Rosie Levine; Jia Li; Ming Li; Christine Lloyd; Susan Lucas; Bin Ma; Donna R Maglott; Elaine R Mardis; Lucy Matthews; Evan Mauceli; John H Mayer; Megan McCarthy; W Richard McCombie; Stuart McLaren; Kirsten McLay; John D McPherson; Jim Meldrim; Beverley Meredith; Jill P Mesirov; Webb Miller; Tracie L Miner; Emmanuel Mongin; Kate T Montgomery; Michael Morgan; Richard Mott; James C Mullikin; Donna M Muzny; William E Nash; Joanne O Nelson; Michael N Nhan; Robert Nicol; Zemin Ning; Chad Nusbaum; Michael J O'Connor; Yasushi Okazaki; Karen Oliver; Emma Overton-Larty; Lior Pachter; Genís Parra; Kymberlie H Pepin; Jane Peterson; Pavel Pevzner; Robert Plumb; Craig S Pohl; Alex Poliakov; Tracy C Ponce; Chris P Ponting; Simon Potter; Michael Quail; Alexandre Reymond; Bruce A Roe; Krishna M Roskin; Edward M Rubin; Alistair G Rust; Ralph Santos; Victor Sapojnikov; Brian Schultz; Jörg Schultz; Matthias S Schwartz; Scott Schwartz; Carol Scott; Steven Seaman; Steve Searle; Ted Sharpe; Andrew Sheridan; Ratna Shownkeen; Sarah Sims; Jonathan B Singer; Guy Slater; Arian Smit; Douglas R Smith; Brian Spencer; Arne Stabenau; Nicole Stange-Thomann; Charles Sugnet; Mikita Suyama; Glenn Tesler; Johanna Thompson; David Torrents; Evanne Trevaskis; John Tromp; Catherine Ucla; Abel Ureta-Vidal; Jade P Vinson; Andrew C Von Niederhausern; Claire M Wade; Melanie Wall; Ryan J Weber; Robert B Weiss; Michael C Wendl; Anthony P West; Kris Wetterstrand; Raymond Wheeler; Simon Whelan; Jamey Wierzbowski; David Willey; Sophie Williams; Richard K Wilson; Eitan Winter; Kim C Worley; Dudley Wyman; Shan Yang; Shiaw-Pyng Yang; Evgeny M Zdobnov; Michael C Zody; Eric S Lander
Journal:  Nature       Date:  2002-12-05       Impact factor: 49.962

2.  Neonatal activation of alcohol-related prenatal memories: impact on the first suckling response.

Authors:  Paula Abate; Elena I Varlinskaya; Sarah J Cheslock; Norman E Spear; Juan Carlos Molina
Journal:  Alcohol Clin Exp Res       Date:  2002-10       Impact factor: 3.455

Review 3.  Epigenetics: role of germ cell imprinting.

Authors:  Marisa S Bartolomei
Journal:  Adv Exp Med Biol       Date:  2003       Impact factor: 2.622

4.  Differences in DNA methylation during oogenesis and spermatogenesis and their persistence during early embryogenesis in the mouse.

Authors:  J P Sanford; H J Clark; V M Chapman; J Rossant
Journal:  Genes Dev       Date:  1987-12       Impact factor: 11.361

5.  CpG islands in vertebrate genomes.

Authors:  M Gardiner-Garden; M Frommer
Journal:  J Mol Biol       Date:  1987-07-20       Impact factor: 5.469

6.  Effect of acute ethanol on serine biosynthesis in liver.

Authors:  L B LaBaume; D K Merrill; G L Clary; R W Guynn
Journal:  Arch Biochem Biophys       Date:  1987-08-01       Impact factor: 4.013

7.  Paternal alcohol exposure affects sperm cytosine methyltransferase messenger RNA levels.

Authors:  Dawn M Bielawski; Fadi M Zaher; David M Svinarich; Ernest L Abel
Journal:  Alcohol Clin Exp Res       Date:  2002-03       Impact factor: 3.455

8.  Differential expression of c-fos in a mouse model of fetal alcohol syndrome.

Authors:  Sarah H Poggi; Katie M Goodwin; Joanna M Hill; Douglas E Brenneman; Elisabetta Tendi; Sergio Schninelli; Catherine Y Spong
Journal:  Am J Obstet Gynecol       Date:  2003-09       Impact factor: 8.661

9.  Temporal and regional changes in DNA methylation in the embryonic, extraembryonic and germ cell lineages during mouse embryo development.

Authors:  M Monk; M Boubelik; S Lehnert
Journal:  Development       Date:  1987-03       Impact factor: 6.868

10.  Non-methylated CpG-rich islands at the human alpha-globin locus: implications for evolution of the alpha-globin pseudogene.

Authors:  A P Bird; M H Taggart; R D Nicholls; D R Higgs
Journal:  EMBO J       Date:  1987-04       Impact factor: 11.598

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  97 in total

1.  Choline supplementation and DNA methylation in the hippocampus and prefrontal cortex of rats exposed to alcohol during development.

Authors:  Nicha K H Otero; Jennifer D Thomas; Christopher A Saski; Xiaoxia Xia; Sandra J Kelly
Journal:  Alcohol Clin Exp Res       Date:  2012-04-17       Impact factor: 3.455

2.  Gene expression changes in C57BL/6J and DBA/2J mice following prenatal alcohol exposure.

Authors:  Chris Downing; Stephen Flink; Maria L Florez-McClure; Thomas E Johnson; Boris Tabakoff; Katerina J Kechris
Journal:  Alcohol Clin Exp Res       Date:  2012-04-24       Impact factor: 3.455

Review 3.  Towards incorporating epigenetic mechanisms into carcinogen identification and evaluation.

Authors:  Zdenko Herceg; Marie-Pierre Lambert; Karin van Veldhoven; Christiana Demetriou; Paolo Vineis; Martyn T Smith; Kurt Straif; Christopher P Wild
Journal:  Carcinogenesis       Date:  2013-06-07       Impact factor: 4.944

4.  Prenatal ethanol exposure disrupts intraneocortical circuitry, cortical gene expression, and behavior in a mouse model of FASD.

Authors:  Hani El Shawa; Charles W Abbott; Kelly J Huffman
Journal:  J Neurosci       Date:  2013-11-27       Impact factor: 6.167

5.  The BAF (BRG1/BRM-Associated Factor) chromatin-remodeling complex exhibits ethanol sensitivity in fetal neural progenitor cells and regulates transcription at the miR-9-2 encoding gene locus.

Authors:  Sasha G Burrowes; Nihal A Salem; Alexander M Tseng; Sridevi Balaraman; Marisa R Pinson; Cadianna Garcia; Rajesh C Miranda
Journal:  Alcohol       Date:  2017-04-07       Impact factor: 2.405

Review 6.  Alcohol effects on the epigenome in the germline: Role in the inheritance of alcohol-related pathology.

Authors:  Lucy G Chastain; Dipak K Sarkar
Journal:  Alcohol       Date:  2017-03-06       Impact factor: 2.405

7.  DNA Methylation program in normal and alcohol-induced thinning cortex.

Authors:  Nail Can Öztürk; Marisol Resendiz; Hakan Öztürk; Feng C Zhou
Journal:  Alcohol       Date:  2017-02-20       Impact factor: 2.405

8.  Disconnect between alcohol-induced alterations in chromatin structure and gene transcription in a mouse embryonic stem cell model of exposure.

Authors:  Kylee J Veazey; Haiqing Wang; Yudhishtar S Bedi; William M Skiles; Richard Cheng-An Chang; Michael C Golding
Journal:  Alcohol       Date:  2017-01-11       Impact factor: 2.405

Review 9.  Evidence from clinical and animal model studies of the long-term and transgenerational impact of stress on DNA methylation.

Authors:  Jennifer Blaze; Tania L Roth
Journal:  Semin Cell Dev Biol       Date:  2015-04-23       Impact factor: 7.727

10.  CB1-receptor knockout neonatal mice are protected against ethanol-induced impairments of DNMT1, DNMT3A, and DNA methylation.

Authors:  Nagaraja N Nagre; Shivakumar Subbanna; Madhu Shivakumar; Delphine Psychoyos; Balapal S Basavarajappa
Journal:  J Neurochem       Date:  2015-01-27       Impact factor: 5.372

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